Hadjiconstantinou M, Neff N H, Zhou L W, Weiss B
Department of Psychiatry, Ohio State University College of Medicine, Columbus 43210, USA.
Neurosci Lett. 1996 Oct 18;217(2-3):105-8.
A D2 dopamine receptor antisense oligodeoxynucleotide was administered intracerebrovetricularly to mice twice on the first day and then once daily for 2 days. The animals were killed 2 h after the last injection, and tyrosine hydroxylase and aromatic L-amino acid decarboxylase activities assayed in the corpus striatum, olfactory tubercle and frontal cortex. Tyrosine hydroxylase activity increased in corpus striatum but not in the olfactory tubercle or in the frontal cortex, while the activity of aromatic L-amino acid decarboxylase increased in all three brain regions. The treatment with the antisense oligomer also elevated the mRNA levels for the two enzymes in the midbrain. In contrast, repeated injection of a vehicle or a random oligomer was without effect on enzyme activity or mRNA D2 antisense oligodeoxynucleotides appear to be selective tools to investigate the role of D2 dopamine receptors in brain.
在第一天给小鼠脑室内注射D2多巴胺受体反义寡脱氧核苷酸两次，然后每天注射一次，持续2天。在最后一次注射后2小时处死动物，测定纹状体、嗅结节和额叶皮质中的酪氨酸羟化酶和芳香族L-氨基酸脱羧酶活性。酪氨酸羟化酶活性在纹状体中增加，但在嗅结节或额叶皮质中未增加，而芳香族L-氨基酸脱羧酶活性在所有三个脑区均增加。反义寡聚物处理还提高了中脑中这两种酶的mRNA水平。相比之下，重复注射载体或随机寡聚物对酶活性或mRNA没有影响。D2反义寡脱氧核苷酸似乎是研究D2多巴胺受体在脑中作用的选择性工具。
