Young E A, Duchemin A M, Neff N H, Hadjiconstantinou M
Neuroscience Program, Ohio State College of Medicine, Columbus 43210, USA.
Eur J Pharmacol. 1998 Sep 11;357(1):15-23. doi: 10.1016/s0014-2999(98)00497-x.
The activity of tyrosine hydroxylase and aromatic L-amino acid decarboxylase in the striatum and their mRNA content in the midbrain were assayed in mice following the intracerebroventricular injection of forskolin or phorbol-12,13-myristic acid (PMA). Control and 1-methyl-1,2,3,6-tetrahydropyridine (MPTP)-lesioned animals were studied. Both forskolin and PMA induced a rapid and transient increase of tyrosine hydroxylase and aromatic L-amino acid decarboxylase activity in the striatum that lasted less than 45 and 60 min, respectively. A second belated increase of striatal tyrosine hydroxylase and aromatic L-amino acid decarboxylase activities was seen only after forskolin, and it was accompanied by a rise of tyrosine hydroxylase and aromatic L-amino acid decarboxylase mRNA in the midbrain. In the MPTP-lesioned mouse, the rise of tyrosine hydroxylase and aromatic L-amino acid decarboxylase following forskolin appeared exaggerated, while the response to PMA was not. These studies suggest that tyrosine hydroxylase and aromatic L-amino acid decarboxylase of striatum can be modulated in parallel by protein kinase A and protein kinase C, and that exaggerated responsiveness to protein kinase A is observed in the partially denervated striatum.
在向小鼠脑室内注射福斯高林或佛波醇-12,13-十四烷酸酯（PMA）后，检测纹状体中酪氨酸羟化酶和芳香族L-氨基酸脱羧酶的活性以及中脑中它们的mRNA含量。对对照组和1-甲基-1,2,3,6-四氢吡啶（MPTP）损伤的动物进行了研究。福斯高林和PMA均诱导纹状体中酪氨酸羟化酶和芳香族L-氨基酸脱羧酶活性快速且短暂增加，分别持续不到45分钟和60分钟。仅在注射福斯高林后，纹状体酪氨酸羟化酶和芳香族L-氨基酸脱羧酶活性出现第二次延迟增加，并伴有中脑中酪氨酸羟化酶和芳香族L-氨基酸脱羧酶mRNA升高。在MPTP损伤的小鼠中，福斯高林引起的酪氨酸羟化酶和芳香族L-氨基酸脱羧酶升高似乎更为明显，而对PMA的反应则不然。这些研究表明，纹状体中的酪氨酸羟化酶和芳香族L-氨基酸脱羧酶可被蛋白激酶A和蛋白激酶C平行调节，并且在部分去神经支配的纹状体中观察到对蛋白激酶A的反应性增强。
