Kankofer M, Podolak M, Fidecki M, Gondek T
Institute of Biochemistry, Faculty of Veterinary Medicine, Agricultural Academy, Lublin, Poland.
Placenta. 1996 Nov;17(8):591-4. doi: 10.1016/s0143-4004(96)80076-2.
Changes in the levels of the activity of two enzymes that neutralize free radicals-glutathione peroxidase [(EC.1.11.1.9), GSH-Px] and superoxide dismutase--[(EC.1.15.1.1), SOD]--in cows with fetal membranes retention (RFM) were studied. The activities were measured in maternal and fetal placental tissues after spontaneous parturition without (group A) and with (group B) retained placenta. GSH-Px activities were higher in group B than in group A (P < 0.05). The activity of this enzyme was lower in the maternal than in the fetal part of the placenta in both groups. The activity of SOD was significantly (P < 0.05) lower in maternal part, and higher in fetal part, of placenta in group B compared with group A. Experiments on defence mechanisms against free radicals in periparturient cows in connection with more frequent sampling, prostaglandins levels, oxidative stress, nutrition and retained placenta are required to further elucidate the role of these enzymes.
研究了患有胎膜滞留（RFM）的奶牛体内两种中和自由基的酶——谷胱甘肽过氧化物酶[（EC.1.11.1.9），GSH-Px]和超氧化物歧化酶——[（EC.1.15.1.1），SOD]活性水平的变化。在自然分娩后，对未发生胎盘滞留（A组）和发生胎盘滞留（B组）的母体和胎儿胎盘组织中的酶活性进行了测定。B组的GSH-Px活性高于A组（P<0.05）。在两组中，该酶在胎盘母体部分的活性均低于胎儿部分。与A组相比，B组胎盘母体部分的SOD活性显著降低（P<0.05），而胎儿部分的SOD活性更高。需要通过更频繁的采样、前列腺素水平、氧化应激、营养状况以及胎盘滞留情况，对围产期奶牛的自由基防御机制进行实验，以进一步阐明这些酶的作用。
