Kankofer M, Wierciński J, Fidecki M
Department of Biochemistry, Faculty of Veterinary Medicine, Agricultural University, Lublin, Poland.
Zentralbl Veterinarmed A. 1998 Sep;45(6-7):337-41. doi: 10.1111/j.1439-0442.1998.tb00836.x.
Hyaluronidase, a proteoglycan-degrading enzyme, may have an influence on collagenolysis in bovine placenta and take part in the separation processes of the placenta in cows. There was, however, no evidence concerning the activity of this enzyme in those tissues. The experiment was performed on cows divided into two groups as follows: A, placenta not retained (n = 16); and B, placenta retained (n = 9). The activity of the enzyme was measured using a spectrophotometric method and zymography. The results showed statistically significant higher activity of hyaluronidase in the maternal and fetal parts of the placenta in cows with a retention of fetal membranes. Further experiments require substrate specificity studies and the presence of regulatory factors of this enzyme in bovine placental tissues.
透明质酸酶是一种蛋白聚糖降解酶，可能对牛胎盘的胶原溶解有影响，并参与奶牛胎盘的分离过程。然而，没有证据表明该酶在这些组织中的活性情况。本实验以奶牛为对象，分为以下两组：A组，胎盘未滞留（n = 16）；B组，胎盘滞留（n = 9）。采用分光光度法和酶谱法测定该酶的活性。结果显示，胎膜滞留的奶牛胎盘母体和胎儿部分的透明质酸酶活性在统计学上显著更高。进一步的实验需要对底物特异性进行研究，并探究牛胎盘组织中该酶的调节因子的存在情况。
