Rizzi M, Nessi C, Bolognesi M, Coda A, Galizzi A
Department of Genetics and Microbiology, A. Buzzati Traverso, University of Pavia, Italy.
Proteins. 1996 Oct;26(2):236-8. doi: 10.1002/(SICI)1097-0134(199610)26:2<236::AID-PROT12>3.0.CO;2-Q.
NAD(+)-synthetase is a ubiquitous enzyme catalyzing the last step in the biosynthesis of NAD+. Mutants of NAD+ synthetase with impaired cellular functions have been isolated, indicating a key role for this enzyme in cellular metabolism. Crystals of the enzyme from Bacillus subtilis suitable for x-ray crystallographic investigation have been grown from polyethylene glycol solutions. Investigation on the structural organization of NAD+ synthetase, an enzyme fundamental for NAD+ biosynthesis, and belonging to the recently characterized amidotransferase enzymatic family, will provide more insight into the catalytic mechanism of deamido-NAD+-->NAD+ conversion, a biosynthetic process that is a potential target for the development of antibiotic compounds against Bacillus sp. and related bacteria.
