DNA损伤剂可在SV40转化的正常人成纤维细胞中诱导产生72-kD热休克蛋白。

DNA-damaging agents induce the 72-kD heat shock protein in SV40 transformed normal human fibroblasts.

作者信息

Muramatsu T, Ohno H, Shirai T, Takahashi A, Ohnishi T

机构信息

Department of Dermatology, Nara Medical University, Japan.

出版信息

J Dermatol. 1996 Sep;23(9):589-93. doi: 10.1111/j.1346-8138.1996.tb02659.x.

DOI:10.1111/j.1346-8138.1996.tb02659.x

PMID:8916656

Abstract

In order to elucidate the involvement of DNA damage in the induction of heat shock proteins (stress proteins), we examined the induction of 72-kD heat shock protein (HSP72) in an SV40-transformed human fibroblast cell line (WI38VA13) which was exposed to various DNA-damaging agents, including 1-(4-amino-2-methyl-5-pyrimidinyl)methyl-3-(2-chloroethyl)-3 -nitro-methylmethane sulfonate, N-methyl-N'-nitro-N-nitrosoguanidine, and 4-nitroquinoline-N-oxide. Induction of HSP72 was detected by the indirect immunofluorescence method using a monoclonal antibody. All the DNA-damaging agents used in this study induced HSP72 on human fibroblasts. This result indicates that DNA damage is one trigger for the induction of HSP72.

摘要

为了阐明DNA损伤在热休克蛋白（应激蛋白）诱导中的作用，我们检测了在暴露于各种DNA损伤剂（包括1-(4-氨基-2-甲基-5-嘧啶基)甲基-3-(2-氯乙基)-3-硝基甲基甲磺酸酯、N-甲基-N'-硝基-N-亚硝基胍和4-硝基喹啉-N-氧化物）的SV40转化人成纤维细胞系（WI38VA13）中72-kD热休克蛋白（HSP72）的诱导情况。使用单克隆抗体通过间接免疫荧光法检测HSP72的诱导。本研究中使用的所有DNA损伤剂均在人成纤维细胞上诱导了HSP72。这一结果表明DNA损伤是诱导HSP72的一个触发因素。