通过cDNA测序分析伪狂犬病病毒基因。

Analysis of pseudorabies virus genes by cDNA sequencing.

作者信息

Ho T Y, Hsiang C Y, Chang T J

机构信息

Department of Veterinary Medicine, National Chung-Hsing University, Taichung, Taiwan, ROC.

出版信息

Gene. 1996 Oct 10;175(1-2):247-51. doi: 10.1016/0378-1119(96)00158-8.

DOI:10.1016/0378-1119(96)00158-8

PMID:8917106

Abstract

Cloning and sequencing of cDNA could provide a complementary approach to functional analysis of the pseudorabies virus (PrV) genome. Using colony hybridization, Southern hybridization, and DNA sequencing, four species of PrV-specific cDNA were identified. Among these four species of PrV-specific cDNA, three unidentified genes, UL26, UL29, and UL31, were mapped and a novel gI-11K bicistronic cDNA was confirmed. Thus, analysis of PrV-specific transcripts provided a way for identifying genes and a foundation to further study the roles of these transcripts in PrV infection.

摘要

克隆和测序cDNA可为伪狂犬病病毒（PrV）基因组的功能分析提供一种互补方法。通过菌落杂交、Southern杂交和DNA测序，鉴定出了四种PrV特异性cDNA。在这四种PrV特异性cDNA中，定位了三个未鉴定的基因，即UL26、UL29和UL31，并证实了一种新的gI-11K双顺反子cDNA。因此，对PrV特异性转录本的分析为鉴定基因提供了一种方法，并为进一步研究这些转录本在PrV感染中的作用奠定了基础。