Dean H J, Cheung A K
National Animal Disease Center, USDA, Ames, Iowa 50010.
Virology. 1994 Aug 1;202(2):962-7. doi: 10.1006/viro.1994.1419.
The DNA sequence of the left end of the pseudorabies virus (PRV) BamHI-G fragment and a short adjacent sequence from the BamHI-C fragment (0.641-0.664 map units) were determined. Two open reading frames were identified and designated PRV UL4 and UL5 because they exhibit amino acid sequence homology to the herpes simplex virus type 1 UL4 and UL5 open reading frames. The PRV UL4 open reading frame codes for a 145-amino acid polypeptide of unknown function. The deduced PRV UL5 gene product is 833 amino acid residues and exhibits sequence characteristics of a helicase, including six sequence motifs which are conserved for a superfamily of helicases. PRV UL5 also contains a putative leucine zipper motif which is not present in other herpesvirus helicase sequences. PRV UL4 and UL5 are transcribed in the opposite orientation with respect to the UL1, UL2, UL3, and UL3.5 gene cluster located at the right end of BamHI-G.
测定了伪狂犬病病毒（PRV）BamHI-G片段左端的DNA序列以及来自BamHI-C片段的一段短相邻序列（0.641-0.664图单位）。鉴定出两个开放阅读框，并命名为PRV UL4和UL5，因为它们与1型单纯疱疹病毒的UL4和UL5开放阅读框具有氨基酸序列同源性。PRV UL4开放阅读框编码一种功能未知的145个氨基酸的多肽。推导的PRV UL5基因产物为833个氨基酸残基，具有解旋酶的序列特征，包括解旋酶超家族保守的六个序列基序。PRV UL5还包含一个假定的亮氨酸拉链基序，这在其他疱疹病毒解旋酶序列中不存在。相对于位于BamHI-G右端的UL1、UL2、UL3和UL3.5基因簇，PRV UL-4和UL5以相反的方向转录。
