Wu J, Ullrich R C, Novotny C P
Department of Miocrobiology and Molecular Genetics, University of Vermont, Burlington 05405, USA.
Mol Gen Genet. 1996 Oct 28;252(6):739-45. doi: 10.1007/BF02173981.
The A alpha mating locus of the woodrotting fungus Schizophyllum commune encodes two multiallelic genes, Y and Z, which regulate the A-pathway of development. The Y alleles contain a homeobox, suggesting that the Y proteins may be DNA-binding regulatory proteins. During mating, development is induced when Y from one mating partner interacts with Z from the other mating partner; self combinations of Y and Z are inactive. Two-hybrid analyses indicate that nonself combinations of Y and Z form heteromultimers and self combinations do not. To understand Y-Z binding and self-nonself recognition further we used mutagenesis and chimeras to identify regions in one allele of Z(Z5) that are involved in these processes. Here we report the results, which broadly define regions in Z5 that are essential for activity, Y-Z binding and Z5 allelic specificity.
木腐真菌裂褶菌的Aα交配位点编码两个多等位基因Y和Z，它们调控A发育途径。Y等位基因含有一个同源异型框，这表明Y蛋白可能是DNA结合调节蛋白。在交配过程中，当一个交配型的Y与另一个交配型的Z相互作用时，发育被诱导；Y和Z的自身组合是无活性的。双杂交分析表明，Y和Z的非自身组合形成异源多聚体，而自身组合则不能。为了进一步了解Y-Z结合和自身-非自身识别，我们使用诱变和嵌合体来鉴定Z（Z5）的一个等位基因中参与这些过程的区域。在此我们报告结果，这些结果广泛地定义了Z5中对于活性、Y-Z结合和Z5等位基因特异性至关重要的区域。
