一种包含具有G·A和A·A非沃森-克里克碱基配对的内部环的弯曲RNA螺旋。
A curved RNA helix incorporating an internal loop with G.A and A.A non-Watson-Crick base pairing.
作者信息
Baeyens K J, De Bondt H L, Pardi A, Holbrook S R
机构信息
Structural Biology Division, Lawrence Berkeley National Laboratory, University of California, Berkeley 94720, USA.
出版信息
Proc Natl Acad Sci U S A. 1996 Nov 12;93(23):12851-5. doi: 10.1073/pnas.93.23.12851.
Abstract
The crystal structure of the RNA dodecamer 5'-GGCC(GAAA)GGCC-3' has been determined from x-ray diffraction data to 2.3-A resolution. In the crystal, these oligomers form double helices around twofold symmetry axes. Four consecutive non-Watson-Crick base pairs make up an internal loop in the middle of the duplex, including sheared G.A pairs and novel asymmetric A.A pairs. This internal loop sequence produces a significant curvature and narrowing of the double helix. The helix is curved by 34 degrees from end to end and the diameter is narrowed by 24% in the internal loop. A Mn2+ ion is bound directly to the N7 of the first guanine in the Watson-Crick region following the internal loop and the phosphate of the preceding residue. This Mn2+ location corresponds to a metal binding site observed in the hammerhead catalytic RNA.
摘要
已根据X射线衍射数据确定了RNA十二聚体5'-GGCC(GAAA)GGCC-3'的晶体结构，分辨率达2.3埃。在晶体中，这些寡聚物围绕二重对称轴形成双螺旋。四个连续的非沃森-克里克碱基对构成双链中间的一个内环，包括剪切的G·A对和新型不对称A·A对。该内环序列使双螺旋产生显著的弯曲和变窄。螺旋从头到尾弯曲34度，内环处直径变窄24%。一个Mn2+离子直接与内环后沃森-克里克区域中第一个鸟嘌呤的N7以及前一个残基的磷酸基团结合。这个Mn2+的位置与在锤头状催化RNA中观察到的金属结合位点相对应。
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