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本文引用的文献

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Structural studies of the 5'-phenazinium-tethered matched and G-A-mismatched DNA duplexes by NMR spectroscopy.通过核磁共振光谱对5'-吩嗪鎓连接的匹配和G-A错配DNA双链体进行结构研究。
J Biochem Biophys Methods. 1993 May;26(2-3):173-236. doi: 10.1016/0165-022x(93)90046-q.
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Proton nuclear Overhauser effect study of the structure of a deoxyoligonucleotide duplex in aqueous solution.水溶液中脱氧寡核苷酸双链体结构的质子核Overhauser效应研究。
Biochemistry. 1983 Apr 12;22(8):2019-25. doi: 10.1021/bi00277a044.
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Sequence-dependent structural variations in two right-handed alternating pyrimidine-purine DNA oligomers in solution determined by nuclear Overhauser enhancement measurements.通过核Overhauser效应测量确定溶液中两种右手交替嘧啶-嘌呤DNA寡聚物的序列依赖性结构变异。
EMBO J. 1983;2(12):2109-15. doi: 10.1002/j.1460-2075.1983.tb01710.x.
4
Proton nuclear magnetic resonance investigation of the conformation and dynamics in the synthetic deoxyribonucleic acid decamers d(ATATCGATAT) and d(ATATGCATAT).合成脱氧核糖核酸十聚体d(ATATCGATAT)和d(ATATGCATAT)的构象与动力学的质子核磁共振研究
Biochemistry. 1983 Dec 6;22(25):5930-42. doi: 10.1021/bi00294a037.
5
Detection of a guanine X adenine base pair in a decadeoxyribonucleotide by proton magnetic resonance spectroscopy.通过质子磁共振光谱法检测脱氧核糖核苷酸中的鸟嘌呤X腺嘌呤碱基对。
Proc Natl Acad Sci U S A. 1983 Jul;80(14):4263-5. doi: 10.1073/pnas.80.14.4263.
6
Assignment of the non-exchangeable proton resonances of d(C-G-C-G-A-A-T-T-C-G-C-G) using two-dimensional nuclear magnetic resonance methods.使用二维核磁共振方法对d（C-G-C-G-A-A-T-T-C-G-C-G）的非交换质子共振进行归属
J Mol Biol. 1983 Dec 15;171(3):319-36. doi: 10.1016/0022-2836(83)90096-7.
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Application of phase sensitive two-dimensional correlated spectroscopy (COSY) for measurements of 1H-1H spin-spin coupling constants in proteins.相敏二维相关光谱法（COSY）在蛋白质中¹H-¹H自旋-自旋耦合常数测量中的应用。
Biochem Biophys Res Commun. 1983 Jun 29;113(3):967-74. doi: 10.1016/0006-291x(83)91093-8.
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Two-dimensional 1H NMR study of the lambda operator site OL1: a sequential assignment strategy and its application.λ 操纵基因位点 OL1 的二维¹H NMR 研究：一种序列归属策略及其应用
Proc Natl Acad Sci U S A. 1984 Jan;81(1):130-4. doi: 10.1073/pnas.81.1.130.
9
A two-dimensional NMR study of the solution structure of a DNA dodecamer comprising the concensus sequence for the specific DNA-binding sites of the glucocorticoid receptor protein.对包含糖皮质激素受体蛋白特异性DNA结合位点共有序列的DNA十二聚体溶液结构的二维核磁共振研究。
Eur J Biochem. 1984 Dec 17;145(3):629-36. doi: 10.1111/j.1432-1033.1984.tb08603.x.
10
Molecular structure of the G.A base pair in DNA and its implications for the mechanism of transversion mutations.DNA中G·A碱基对的分子结构及其对颠换突变机制的影响。
Proc Natl Acad Sci U S A. 1986 Apr;83(8):2402-6. doi: 10.1073/pnas.83.8.2402.

对模拟核酶的寡脱氧核糖核苷酸双链体中G:A错配的核磁共振研究。

NMR studies of G:A mismatches in oligodeoxyribonucleotide duplexes modelled after ribozymes.

作者信息

Katahira M, Sato H, Mishima K, Uesugi S, Fujii S

机构信息

Department of Bioengineering, Faculty of Engineering, Yokohama National University, Japan.

出版信息

Nucleic Acids Res. 1993 Nov 25;21(23):5418-24. doi: 10.1093/nar/21.23.5418.

DOI:10.1093/nar/21.23.5418
PMID:8265358
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC310580/
Abstract

The structures of two oligodeoxyribonucleotide duplexes, the base sequences of which were modelled after both a hammerhead ribozyme and a small metalloribozyme, were studied by NMR. Both duplexes contain adjacent G:A mismatches; one has a PyGAPu:PyGAPu sequence and the other a PyGAPy:PuGAPu sequence. It is concluded on the basis of many characteristic NOEs that in both duplexes G:A base pairs are formed in the unique 'sheared' form, where an amino proton instead of an imino proton of G is involved in the hydrogen bonding, and G and A bases are arranged 'side by side' instead of 'head to head'. A photo-CIDNP experiment, which gives unique and independent information on the solvent accessibility of nucleotide bases, also supports G:A base pairing rather than a bulged-out structure of G and A residues. This is the first demonstration that not only the PyGAPu:PyGAPu sequence but also the PyGAPy:PuGAPu sequence can form the unique sheared G:A base pairs. Taking the previous studies on G:A mismatches into account, the idea is suggested that a PyGA:GAPu sequence is a minimum and essential element for the formation of the sheared G:A base pairs. The sheared G:A base pairs in the PyGAPu:PyGAPu sequence are suggested to be more stable than those in the PyGAPy:PuGAPu sequence. This is explained rationally by the idea proposed above.

摘要

通过核磁共振研究了两个寡脱氧核糖核苷酸双链体的结构，其碱基序列是根据锤头状核酶和小金属核酶构建的。两个双链体均含有相邻的G:A错配；一个具有PyGAPu:PyGAPu序列，另一个具有PyGAPy:PuGAPu序列。基于许多特征性的核Overhauser效应（NOE）得出结论，在两个双链体中，G:A碱基对均以独特的“剪切”形式形成，其中参与氢键形成的是G的氨基质子而非亚氨基质子，并且G和A碱基是“并排”排列而非“头对头”排列。光化学诱导动态核极化（photo-CIDNP）实验能够提供关于核苷酸碱基溶剂可及性的独特且独立的信息，该实验也支持G:A碱基配对而非G和A残基的突出结构。这是首次证明不仅PyGAPu:PyGAPu序列而且PyGAPy:PuGAPu序列都能形成独特的剪切G:A碱基对。考虑到先前对G:A错配的研究，有人提出PyGA:GAPu序列是形成剪切G:A碱基对的最小且必需的元件。PyGAPu:PyGAPu序列中的剪切G:A碱基对被认为比PyGAPy:PuGAPu序列中的更稳定。上述观点对此进行了合理的解释。