RGS-r是一种视网膜特异性RGS蛋白，它与转导素的中间构象结合并增强循环利用。
RGS-r, a retinal specific RGS protein, binds an intermediate conformation of transducin and enhances recycling.
作者信息
Chen C K, Wieland T, Simon M I
机构信息
Division of Biology, California Institute of Technology, Pasadena 91125, USA.
出版信息
Proc Natl Acad Sci U S A. 1996 Nov 12;93(23):12885-9. doi: 10.1073/pnas.93.23.12885.
Abstract
G proteins regulate intracellular signaling by coupling a cycle of guanine nucleotide binding and hydrolysis to transient changes of cellular functions. The mechanisms that control the recycling of transducin, the "pacesetting" G protein that regulates mammalian phototransduction, are unclear. We show that a novel retinal specific RGS-motif protein specifically binds to an intermediate conformation involved in GTP hydrolysis by transducin and accelerates phosphate release and the recycling of transducin. This specific interaction further rationalizes the kinetics of the phototransduction cascade and provides a general hypothesis to explain the mechanism of interaction of RGS proteins with other G proteins.
摘要
G蛋白通过将鸟嘌呤核苷酸结合与水解的循环与细胞功能的瞬时变化偶联来调节细胞内信号传导。控制转导素（调节哺乳动物光转导的“定步速”G蛋白）循环利用的机制尚不清楚。我们发现一种新的视网膜特异性RGS基序蛋白特异性结合转导素参与GTP水解的中间构象，并加速磷酸释放和转导素的循环利用。这种特异性相互作用进一步阐明了光转导级联反应的动力学，并为解释RGS蛋白与其他G蛋白相互作用的机制提供了一个普遍的假设。
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