聚合酶链反应中DNA复制的效率。
Efficiency of DNA replication in the polymerase chain reaction.
作者信息
Stolovitzky G, Cecchi G
机构信息
Center for Studies in Physics and Biology, Rockefeller University, New York, NY 10021, USA.
出版信息
Proc Natl Acad Sci U S A. 1996 Nov 12;93(23):12947-52. doi: 10.1073/pnas.93.23.12947.
Abstract
A detailed quantitative kinetic model for the polymerase chain reaction (PCR) is developed, which allows us to predict the probability of replication of a DNA molecule in terms of the physical parameters involved in the system. The important issue of the determination of the number of PCR cycles during which this probability can be considered to be a constant is solved within the framework of the model. New phenomena of multimodality and scaling behavior in the distribution of the number of molecules after a given number of PCR cycles are presented. The relevance of the model for quantitative PCR is discussed, and a novel quantitative PCR technique is proposed.
摘要
我们建立了一个用于聚合酶链反应（PCR）的详细定量动力学模型，该模型使我们能够根据系统中涉及的物理参数预测DNA分子复制的概率。在该模型框架内解决了确定PCR循环次数的重要问题，在此期间该概率可被视为常数。文中展示了给定数量的PCR循环后分子数分布中的多峰性和标度行为等新现象。讨论了该模型在定量PCR中的相关性，并提出了一种新颖的定量PCR技术。
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