有丝分裂原激活蛋白激酶信号传导成分发生突变的白色念珠菌杂合子和纯合子菌株在菌丝发育方面存在缺陷。
Candida albicans strains heterozygous and homozygous for mutations in mitogen-activated protein kinase signaling components have defects in hyphal development.
作者信息
Köhler J R, Fink G R
机构信息
Whitehead Institute for Biomedical Research, Cambridge, MA, USA.
出版信息
Proc Natl Acad Sci U S A. 1996 Nov 12;93(23):13223-8. doi: 10.1073/pnas.93.23.13223.
Abstract
The Candida albicans genes, CST20 and HST7, were cloned by their ability to suppress the mating defects of Saccharomyces cerevisiae mutants in the ste20 and ste7 genes, which code for elements of the mating mitogenactivated protein (MAP) kinase pathway. These Candida genes are both structural and functional homologs of the cognate Saccharomyces genes. The pattern of suppression in Saccharomyces is related to their presumptive position in the MAP kinase cascade. Null alleles of these genes were constructed in Candida. The Candida homozygous null mutants are defective in hyphal formation on some media, but are still induced to form hyphae by serum, showing that serum induction of hyphae is independent of the MAP kinase cascade. The Candida heterozygotes CST20/cst20 and HST7/hst7 are also defective in hyphal formation. This lack of dominance of the wild-type allele suggests that gene dosage is important in Candida.
摘要
白色念珠菌基因CST20和HST7是通过其抑制酿酒酵母ste20和ste7基因突变体交配缺陷的能力而克隆得到的，这两个基因编码交配丝裂原活化蛋白（MAP）激酶途径的元件。这些念珠菌基因是同源酿酒酵母基因的结构和功能同源物。酿酒酵母中的抑制模式与其在MAP激酶级联反应中的假定位置有关。在念珠菌中构建了这些基因的无效等位基因。念珠菌纯合无效突变体在某些培养基上形成菌丝的过程存在缺陷，但仍可被血清诱导形成菌丝，这表明血清诱导菌丝形成与MAP激酶级联反应无关。念珠菌杂合子CST20/cst20和HST7/hst7在菌丝形成方面也存在缺陷。野生型等位基因缺乏显性表明基因剂量在念珠菌中很重要。
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