Humphries S E, Talmud P J, Cox C, Sutherland W, Mann J
Department of Medicine, UCL Medical School, London, UK.
QJM. 1996 Sep;89(9):671-80. doi: 10.1093/qjmed/89.9.671.
We examined the role of common genetic variation in determining the consistency and magnitude of change in plasma total cholesterol (TC) levels in response to two separate changes from a high-saturated (SFA) to a low-saturated/high-polyunsaturated-fat (PUFA) diet, in a group of free-living healthy men and women. Consistent responders were defined as those whose mean difference in the change in TC was within one SD of the mean for all participants, and the remainder were defined as variable responders. DNA was obtained from 55 individuals and genotype determined at the apolipoprotein (apo) B locus (signal peptide, SP), apoCIII (C1100-T) and lipoprotein lipase (LPL) gene loci (HindIII). In the 38 consistent responders, the apoBSP24 allele was significantly more common than in the 17 individuals with a variable response (0.29 vs. 0.12; p < 0.05). No other polymorphism showed a significant frequency difference between groups. In the group as a whole, the correlation between the change in TC level in response to the first and second dietary change was 0.28 (p = 0.05), but those with one or more apoB SP24 alleles and those with the apoCIII genotype CC had a significantly higher correlation than those with other genotypes (0.46 (p = 0.05) vs. 0.12 (NS) and 0.31 (p = 0.05) vs. 0.02 (NS), respectively). In the group as a whole, mean response left TC 10% higher on the SFA than on the PUFA diet, and neither apoB nor apoCIII genotypes affected the magnitude of this response. However, individuals with the LPL HindIII genotype H+ H+ had a significantly smaller change in mean TC in response to diet than those with one or more H- allele (9.3% vs. 14.4%; p = 0.03). Thus variation at the apoB and apoCIII loci affects the consistency of response to change in dietary fat content, while variation at the LPL gene locus affects magnitude of response.
我们在一组自由生活的健康男性和女性中，研究了常见基因变异在决定血浆总胆固醇（TC）水平对从高饱和脂肪（SFA）饮食到低饱和/高多不饱和脂肪（PUFA）饮食的两种不同变化的反应一致性和变化幅度方面的作用。一致性反应者被定义为其TC变化的平均差异在所有参与者平均值的一个标准差范围内的个体，其余个体被定义为可变反应者。从55名个体中获取DNA，并在载脂蛋白（apo）B基因座（信号肽，SP）、apoCIII（C1100 - T）和脂蛋白脂肪酶（LPL）基因座（HindIII）处确定基因型。在38名一致性反应者中，apoBSP24等位基因比17名可变反应个体中更为常见（0.29对0.12；p < 0.05）。没有其他多态性在两组之间显示出显著的频率差异。在整个组中，对第一次和第二次饮食变化的TC水平变化之间的相关性为0.28（p = 0.05），但具有一个或多个apoB SP24等位基因的个体以及具有apoCIII基因型CC的个体的相关性显著高于其他基因型个体（分别为0.46（p = 0.05）对0.12（无显著性差异）和0.31（p = 0.05）对0.02（无显著性差异））。在整个组中，平均反应使SFA饮食时的TC比PUFA饮食时高10%，apoB和apoCIII基因型均不影响这种反应的幅度。然而，具有LPL HindIII基因型H + H + 的个体对饮食的平均TC变化显著小于具有一个或多个H - 等位基因的个体（9.3%对14.4%；p = 0.03）。因此，apoB和apoCIII基因座的变异影响对饮食脂肪含量变化的反应一致性，而LPL基因座的变异影响反应幅度。
