Threlfall E J, Hampton M D, Schofield S L, Ward L R, Frost J A, Rowe B
Commun Dis Rep CDR Rev. 1996 Oct 11;6(11):R155-9.
Human isolates of multiresistant Salmonella typhimurium definitive phage type (DT) 104 in England and Wales are currently second in number only to those of S. enteritidis phage type 4. Differentiation of strains is essential in epidemiological investigations and the value of one method, plasmid profile typing, has been assessed in a study of 600 isolates of S. typhimurium DT 104 with multiresistant antibiograms (R-types) ACSSuT, ACSSuTCp and ACSSuTTm from humans, food animals, human food, pets, and animal feed made in England and Wales from January 1990 to April 1996. Twenty plasmid profile (PP) types have been identified in isolates of R-type ACSSuT and ACSSuTCp. One profile type, with a single plasmid of 60 megadaltons-PP type A-has predominated, but identification of PP type has proved useful in some epidemiological investigations. A further four PP types have been identified in isolates of DT 104 R-type ACSSuTTm, in which resistance to trimethoprim is encoded by a plasmid of 4.6 megadaltons and the two commonest PP types are related to those also common in DT 104 R-type ACSSuT. Methods of differentiating within the commonest profile type are now needed.
在英格兰和威尔士，多重耐药鼠伤寒沙门氏菌定型噬菌体类型（DT）104的人类分离株数量目前仅次于肠炎沙门氏菌噬菌体类型4的分离株。菌株鉴别在流行病学调查中至关重要，在一项研究中评估了一种方法——质粒图谱分型的价值，该研究涉及1990年1月至1996年4月在英格兰和威尔士采集的600株具有多重耐药抗菌谱（R型）ACSSuT、ACSSuTCp和ACSSuTTm的鼠伤寒沙门氏菌DT 104分离株，这些分离株来自人类、食用动物、人类食物、宠物以及动物饲料。在R型ACSSuT和ACSSuTCp的分离株中已鉴定出20种质粒图谱（PP）类型。一种图谱类型，即带有一个60兆道尔顿的单个质粒——PP A型——占主导地位，但已证明PP类型的鉴定在一些流行病学调查中很有用。在DT 104 R型ACSSuTTm的分离株中又鉴定出另外4种PP类型，其中对甲氧苄啶的耐药性由一个4.6兆道尔顿的质粒编码，两种最常见的PP类型与DT 104 R型ACSSuT中也常见的类型相关。现在需要在最常见的图谱类型中进行区分的方法。
