el Zayat E A
Parasitology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt.
J Egypt Soc Parasitol. 1996 Dec;26(3):677-85.
Indirect haemagglutination test (IHA) commercially available kits (Fumouze) for diagnosis of fascioliasis and schistosomiasis were evaluated for cross reactivity. Sera of 85 selected schistosomiasis patients (Group I) were tested for schistosomiasis titers employing purified Schistosomiasis mansoni egg antigen. Sensitivity of the test was 75.3%. Positive sera (Group II: 64 sera) were retested using kits for fascioliasis employing purified adult Fasciola hepatica antigen to detect any cross reactions. Specificity was 96.9%. In addition, thirty eight (38) sera of selected fascioliasis cases (Group III) were retested using IHA kits for schistosomiasis. Specificity was 94.7%. It was found that IHA employing purified specific antigens both for schistosomiasis and fascioliasis gives good specificities and helps to avoid cross reactions between the two serious endemic trematode infections offering reliable diagnosis.
对市售用于诊断肝片吸虫病和血吸虫病的间接血凝试验（IHA）试剂盒（Fumouze）进行交叉反应性评估。采用纯化的曼氏血吸虫虫卵抗原，对85例选定的血吸虫病患者血清（第一组）进行血吸虫病滴度检测。该检测的灵敏度为75.3%。使用采用纯化的肝片吸虫成虫抗原的肝片吸虫病试剂盒对阳性血清（第二组：64份血清）进行重新检测，以检测任何交叉反应。特异性为96.9%。此外，使用血吸虫病IHA试剂盒对38例选定的肝片吸虫病病例血清（第三组）进行重新检测。特异性为94.7%。结果发现，采用针对血吸虫病和肝片吸虫病的纯化特异性抗原的IHA具有良好的特异性，有助于避免两种严重的地方性吸虫感染之间的交叉反应，提供可靠的诊断。
