Stintzi A, Evans K, Meyer J M, Poole K
Department of Microbiology and Immunology, Queen's University, Kingston, Ont., Canada.
FEMS Microbiol Lett. 1998 Sep 15;166(2):341-5. doi: 10.1111/j.1574-6968.1998.tb13910.x.
Cell density-dependent gene expression in Pseudomonas aeruginosa is controlled, in part, by the quorum-sensing regulator LasR. lasR null mutants exhibited a reproducible 2-fold decrease in production of the catecholate-hydroxamate siderophore pyoverdine during grown under iron-limiting conditions. Similarly, lasI mutants defective in the biosynthesis of the autoinducer PAI-1 also exhibited a 2-fold decrease in pyoverdine production which could be largely restored upon addition of exogenous PAI-1. lasR mutants were not altered with respect to expression of the pvdD gene involved in the synthesis of the peptide portion of pyoverdine, indicating that some other pyoverdine biosynthetic gene(s) were affected by the LasRI status of the cell. This represents the first report of quorum-sensing regulation of siderophore production in bacteria and highlights the fact that cell density, while not an essential signal for pyoverdine expression, does enhance production of this siderophore.
铜绿假单胞菌中细胞密度依赖性基因表达部分受群体感应调节因子LasR控制。在铁限制条件下生长时，lasR基因敲除突变体的儿茶酚 - 异羟肟酸铁载体绿脓菌素产量可重复性降低2倍。同样，在自诱导物PAI - 1生物合成方面存在缺陷的lasI突变体，其绿脓菌素产量也降低了2倍，添加外源PAI - 1后产量可在很大程度上恢复。lasR突变体中参与绿脓菌素肽段合成的pvdD基因表达没有改变，这表明其他一些绿脓菌素生物合成基因受到细胞LasRI状态的影响。这是关于细菌中铁载体产生的群体感应调节的首次报道，并突出了这样一个事实，即细胞密度虽然不是绿脓菌素表达的必需信号，但确实会增强这种铁载体的产生。
