Hsia R C, Bavoil P M
Department of Microbiology and Immunology, University of Rochester Medical Center, NY 14642, USA.
Gene. 1996 Oct 17;176(1-2):163-9. doi: 10.1016/0378-1119(96)00240-5.
The nucleotide sequence of nearly 6 kb of genomic DNA located immediately upstream of the omp3-omp2 operon of Chlamydia psittaci strain GPIC was obtained, revealing four significant open reading frames (ORFs), named ORF1, ORF2, ORF4 and ORF5. Searches for homologous sequences in the GenBank/EMBL databases have revealed that: (a) the open-ended ORF1 putatively encodes an homolog of RecJ of Escherichia coli, thought to be required for RecBCD-independent and conjugational recombination, and for UV repair; (b) the predicted translation product of ORF4 is highly homologous to the putative product of EUO, a previously described ORF of avian C. psittaci strain 6BC which is preferentially transcribed early during the life cycle; and (c) ORF5 putatively encodes an homolog of bacterial glutamyl-tRNA synthetases. This analysis establishes the genetic linkage of late (omp3-omp2) and of a proposed early (EUO) genes in Chlamydia.
获得了鹦鹉热衣原体菌株GPIC的omp3 - omp2操纵子上游紧邻的近6 kb基因组DNA的核苷酸序列，发现了四个重要的开放阅读框（ORF），分别命名为ORF1、ORF2、ORF4和ORF5。在GenBank/EMBL数据库中搜索同源序列发现：（a）开放式ORF1可能编码大肠杆菌RecJ的同源物，RecJ被认为是RecBCD非依赖性和接合重组以及紫外线修复所必需的；（b）ORF4的预测翻译产物与EUO的推定产物高度同源，EUO是先前描述的禽鹦鹉热衣原体菌株6BC的一个ORF，在生命周期早期优先转录；（c）ORF5可能编码细菌谷氨酰胺 - tRNA合成酶的同源物。该分析确定了衣原体中晚期（omp3 - omp2）和一个假定的早期（EUO）基因的遗传连锁关系。
