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本文引用的文献

1
Detection of Mycoplasma pneumoniae by polymerase chain reaction and nonradioactive hybridization in microtiter plates.采用聚合酶链反应及微量滴定板非放射性杂交技术检测肺炎支原体
J Clin Microbiol. 1993 May;31(5):1088-94. doi: 10.1128/jcm.31.5.1088-1094.1993.
2
Improved microplate immunoenzymatic assay of PCR products for rapid detection of Mycoplasma pneumoniae.用于快速检测肺炎支原体的PCR产物改良微孔板免疫酶测定法。
Mol Cell Probes. 1995 Feb;9(1):25-31. doi: 10.1016/s0890-8508(95)90947-8.
3
Small-scale DNA preparation for rapid genetic identification of Campylobacter species without radioisotope.用于弯曲杆菌属物种快速基因鉴定的无放射性同位素的小规模DNA制备方法。
Microbiol Immunol. 1988;32(2):141-50. doi: 10.1111/j.1348-0421.1988.tb01373.x.
4
Quantification of picogram levels of specific DNA immobilized in microtiter wells.对固定在微量滴定板孔中的特定皮克级DNA进行定量分析。
FEBS Lett. 1985 Apr 22;183(2):379-82. doi: 10.1016/0014-5793(85)80814-0.
5
Non-radioactive hybridization probes prepared by the chemical labelling of DNA and RNA with a novel reagent, photobiotin.通过用新型试剂光生物素对DNA和RNA进行化学标记制备的非放射性杂交探针。
Nucleic Acids Res. 1985 Feb 11;13(3):745-61. doi: 10.1093/nar/13.3.745.
6
Microplate hybridization of amplified viral DNA segment.扩增病毒DNA片段的微孔板杂交
J Clin Microbiol. 1990 Jun;28(6):1469-72. doi: 10.1128/jcm.28.6.1469-1472.1990.
7
Covalent immobilization of DNA onto polystyrene microwells: the molecules are only bound at the 5' end.将DNA共价固定到聚苯乙烯微孔板上：分子仅在5'端结合。
Anal Biochem. 1991 Oct;198(1):138-42. doi: 10.1016/0003-2697(91)90518-x.

改进DNA在微孔板上的固定以用于DNA-DNA杂交。

Improved immobilization of DNA to microwell plates for DNA-DNA hybridization.

作者信息

Hirayama H, Tamaoka J, Horikoshi K

机构信息

Deep Star Group, Japan Marine Science and Technology Center, Yokosuka, Kanagawa.

出版信息

Nucleic Acids Res. 1996 Oct 15;24(20):4098-9. doi: 10.1093/nar/24.20.4098.

DOI:10.1093/nar/24.20.4098
PMID:8918820
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC146194/
Abstract

An improved and simplified protocol for DNA immobilization was developed to enhance DNA-DNA hybridization on microwell plates. Target DNA was immobilized by simple dry-adsorption. Efficiencies of DNA immobilization and retention were enhanced 1.4-6.5 times and 4.2-19.6 times, respectively, compared with a conventional method. The overall hybridization efficiency was increased 3.1-5.2 times. This simple new protocol can reduce the consumption of scarce DNA samples.

摘要

为了增强微孔板上的DNA-DNA杂交，开发了一种改进和简化的DNA固定方案。通过简单的干吸附固定靶DNA。与传统方法相比，DNA固定和保留效率分别提高了1.4至6.5倍和4.2至19.6倍。整体杂交效率提高了3.1至5.2倍。这种简单的新方案可以减少稀缺DNA样本的消耗。