Reipert B M, Allan D, Dexter T M
Cancer Research Campaign Department of Physics & Instrumentation, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, UK.
Growth Factors. 1996;13(3-4):205-17. doi: 10.3109/08977199609003222.
Recent reports indicate an increased risk of acute myeloid leukaemia in children exposed to extremely low frequency magnetic fields (ELFMFs) emitted by high voltage power lines, suggesting that ELFMFs may act as weak tumour promoters. We have investigated possible interactions of weak ELFMFs with primitive haemopoietic cells in vitro using the multipotential progenitor cell line FDCP-mix(A4). We have determined the proliferative activity and clonogenic potential of cells under both optimal and sub-optimal growth conditions and exposed to either ambient laboratory ELFMFs or three other ELFMF regimes representative of those produced by high voltage power lines: nulled fields, Ca2+-ion cyclotron resonance conditions at 50 Hz, and vertical 50 Hz fields of 6 muT(RMS). Using exposures of 1, 4, 7 and 21 days, we found no significant alteration of growth rate, cell-cycle state or clonogenic efficiency indicating that neither the proliferation nor self-renewal of multipotential FDCP-mix(A4) cells was perturbed.
近期报告显示，暴露于高压输电线发出的极低频磁场（ELFMFs）中的儿童患急性髓性白血病的风险增加，这表明ELFMFs可能作为弱肿瘤促进剂起作用。我们使用多能祖细胞系FDCP-mix(A4)在体外研究了弱ELFMFs与原始造血细胞之间可能的相互作用。我们已经确定了在最佳和次优生长条件下，以及暴露于实验室环境ELFMFs或代表高压输电线产生的其他三种ELFMF模式（零场、50Hz的Ca2+离子回旋共振条件和均方根值为6μT的垂直50Hz场）下细胞的增殖活性和克隆形成潜力。通过1、4、7和21天的暴露，我们发现生长速率、细胞周期状态或克隆形成效率没有显著改变，这表明多能FDCP-mix(A4)细胞的增殖和自我更新均未受到干扰。
