Reipert B M, Allan D, Reipert S, Dexter T M
Cancer Research Campaign Department of Physics & Instrumentation, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, UK.
Life Sci. 1997;61(16):1571-82. doi: 10.1016/s0024-3205(97)00736-4.
Epidemiological studies have indicated a modestly increased risk for the development of acute myeloid leukaemia in children who live close to high-voltage power-lines. Recent evidence has suggested that a common property shared by a number of known and suspected tumour promoters is their ability to block the process of apoptosis. Therefore, one possible mechanistic explanation for the apparent leukaemogenic effect of weak, low-frequency magnetic fields, such as emitted by power-lines and electrical appliances, would be their expression of tumour-promoting activity by interfering with the regulation of apoptosis in multipotent haemopoietic progenitor cells. In order to test this hypothesis, we have employed the well-characterized multipotential haemopoietic progenitor cell line FDCP-mix(A4). These cells are non-leukaemic and undergo apoptosis when deprived of appropriate growth factors such as Interleukin-3. We have tested a series of different regimes of weak, low-frequency magnetic fields: nulled fields, Ca2+-ion cyclotron resonance conditions at 50 Hz, and vertical 50 Hz fields of 6 microT(RMS), 1 mT(RMS) and 2 mT(RMS), exposing the cells for 2 hours, 24 hours, 4 days or 7 days under various culture conditions. We have not seen any significant alteration in apoptosis induced by any of the exposure regimes tested. We therefore conclude that the regulation of viability and apoptosis in FDCP-mix(A4) cells is not disturbed by weak magnetic fields of the magnitude and type indicated.
流行病学研究表明，居住在高压电线附近的儿童患急性髓系白血病的风险略有增加。最近的证据表明，许多已知和疑似肿瘤促进剂的一个共同特性是它们阻断细胞凋亡过程的能力。因此，对于电线和电器发出的微弱低频磁场明显的致白血病作用，一种可能的机制解释是，它们通过干扰多能造血祖细胞中细胞凋亡的调节来表现出肿瘤促进活性。为了验证这一假设，我们使用了特征明确的多能造血祖细胞系FDCP-mix(A4)。这些细胞是非白血病细胞，在缺乏适当生长因子（如白细胞介素-3）时会发生凋亡。我们测试了一系列不同的微弱低频磁场条件：零磁场、50赫兹的钙离子回旋共振条件以及均方根值为6微特斯拉、1毫特斯拉和2毫特斯拉的垂直50赫兹磁场，在各种培养条件下将细胞暴露2小时、24小时、4天或7天。我们没有观察到任何测试暴露条件诱导的细胞凋亡有任何显著变化。因此，我们得出结论，FDCP-mix(A4)细胞的活力和凋亡调节不受所示强度和类型的弱磁场干扰。
