Petersen T I, Kissmeyer-Nielsen P, Flyvbjerg A, Laurberg S, Christensen H
Department of Surgery, University Hospital of Aarhus, Denmark.
Int J Colorectal Dis. 1996;11(1):19-24. doi: 10.1007/BF00418850.
The gastrointestinal tract is highly responsive to the tropic effect of growth hormone (GH). GH stimulates the healing of colonic anastomoses either directly or through insulin-like growth factor I (IGF-I) since specific GH receptor as well as IGF-I receptor have been demonstrated in colon.
To determine whether exogenous treatment with IGF-I could stimulate the healing of left colonic anastomoses in rats.
After colonic anastomotic operations adult rats were randomised to treatment with either IGF-I (500 mu g per day) or vehicle (controls). Anastomotic breaking strength and collagen deposition were determined at day after surgery.
IGF-I treatment increased the anastomotic collagen content by 23% compared with controls. This resulted in a lower extensibility of the anastomosis (P < 0.05), whereas the anastomotic breaking strength did not differ between groups. The treatment resulted in a 3 fold increase in serum IGF-I of IGF-I treated rats, compared to controls. The postoperative body weight increased by 5% in the IGF-I rats from day 0 to day 3, while the control group had a weight loss of 2% in the same period (P < 0.001).
Treatment of colon-operated rats with IGF-I increased the postoperative body weight and stimulates the collagen deposition of left colonic anastomoses, whereas the anastomotic strength may be unaffected by IGF-I treatment.
胃肠道对生长激素（GH）的促生长作用高度敏感。GH可直接或通过胰岛素样生长因子I（IGF-I）刺激结肠吻合口愈合，因为已在结肠中证实了特异性GH受体以及IGF-I受体。
确定外源性给予IGF-I是否能刺激大鼠左半结肠吻合口的愈合。
成年大鼠进行结肠吻合手术后，随机分为IGF-I治疗组（每天500μg）和对照组（给予赋形剂）。在术后第天测定吻合口破裂强度和胶原蛋白沉积量。
与对照组相比，IGF-I治疗使吻合口胶原蛋白含量增加了23%。这导致吻合口的伸展性降低（P<0.05），而两组间吻合口破裂强度无差异。与对照组相比，IGF-I治疗组大鼠血清IGF-I增加了3倍。从术后第0天到第3天，IGF-I治疗组大鼠体重增加了5%，而同期对照组体重下降了2%（P<0.001）。
用IGF-I治疗结肠手术大鼠可增加术后体重，并刺激左半结肠吻合口的胶原蛋白沉积，而IGF-I治疗可能不影响吻合口强度。
