Nourbakhsh F, Uliana S R, Smith D F
Department of Biochemistry, Imperial College of Science, Technology and Medicine, London, UK.
Mol Biochem Parasitol. 1996 Feb-Mar;76(1-2):201-13. doi: 10.1016/0166-6851(95)02559-6.
This paper describes the isolation and characterisation of the meta 1 gene from Leishmania major, that is expressed predominantly in infective metacyclic parasites. The termini of the major 2-kb transcript derived from this gene have been mapped and polypyrimidine sequences implicated in RNA processing located in the flanking regions. The meta 1 gene is conserved in both Old and New World Leishmania species and codes for a putative protein of 112 amino acids. Antibodies raised against a recombinant protein expressed from this open reading frame recognise an 11.5-kDa protein in metacyclic cell lysates and this molecule localises to the region of the flagellar pocket by indirect immunofluorescence.
本文描述了来自硕大利什曼原虫的meta 1基因的分离和特性，该基因主要在感染性循环后期寄生虫中表达。已绘制了源自该基因的主要2 kb转录本的末端图谱，并确定了位于侧翼区域参与RNA加工的多嘧啶序列。meta 1基因在新旧世界利什曼原虫物种中均保守，编码一种推定的112个氨基酸的蛋白质。针对从该开放阅读框表达的重组蛋白产生的抗体可识别循环后期细胞裂解物中的11.5 kDa蛋白，并且该分子通过间接免疫荧光定位于鞭毛袋区域。
