Carman A S, Kuan S S, Ware G M, Umrigar P P, Miller K V, Guerrero H G
Natural Toxins Research Center, U.S. Food and Drug Administration, New Orleans, LA 70122-3896, USA.
J AOAC Int. 1996 Mar-Apr;79(2):456-64.
Immunoaffinity column-based sample preparation procedures for determination of aflatoxins B1, B2, G1, and G2 in several food matrixes and aflatoxin M1 in milk have been automated by using flexible automation, or robotics. Components used to assemble the system were purchased commercially or developed and built in-house. A liquid-level sensor developed in-house to assist elution of the immunoaffinity column is described. After immunoaffinity column cleanup, aflatoxins are separated by reversed-phase liquid chromatography and determined by fluorescence without derivatization. Mean recoveries of aflatoxins B1, B2, and G1 added to corn and nuts at 9-36 ng/g total aflatoxins were > 85% (coefficient of variation [CV] = 16%). Recoveries of aflatoxin G2 averaged 50% (CV = 28%). Recoveries of aflatoxin M1 added to milk at 0.12-0.50 ng/mL averaged 78% (CV = 19%). The ability of the automated system to reproduce its results is demonstrated by the fact that the CV of replicate assays is generally better than 10%. Comparability between the automated procedure and the AOAC official method is demonstrated.
基于免疫亲和柱的样品制备程序，用于测定多种食品基质中的黄曲霉毒素B1、B2、G1和G2以及牛奶中的黄曲霉毒素M1，已通过使用灵活自动化或机器人技术实现了自动化。用于组装该系统的组件是通过商业采购或在内部开发和制造的。本文描述了一种在内部开发的用于辅助免疫亲和柱洗脱的液位传感器。经过免疫亲和柱净化后，黄曲霉毒素通过反相液相色谱法分离，并通过荧光法测定，无需衍生化。添加到玉米和坚果中、总黄曲霉毒素含量为9 - 36 ng/g的黄曲霉毒素B1、B2和G1的平均回收率> 85%（变异系数[CV] = 16%）。黄曲霉毒素G2的回收率平均为50%（CV = 28%）。添加到牛奶中、含量为0.12 - 0.50 ng/mL的黄曲霉毒素M1的回收率平均为78%（CV = 19%）。重复测定的CV通常优于10%，这证明了自动化系统重现其结果的能力。本文还证明了自动化程序与AOAC官方方法之间的可比性。
