Scott P M, Lawrence G A
Health Canada, Health Protection Branch, Ottawa, ON, Canada.
J AOAC Int. 1997 Nov-Dec;80(6):1229-34.
Aflatoxins B1, B2, G1, and G2 were determined at parts-per-trillion levels in beer by immunoaffinity column cleanup and reversed-phase liquid chromatography (LC) with fluorescence detection after trifluoroacetic acid derivatization. Silanized vials were necessary for the evaporation step in order to obtain good recoveries of aflatoxins from spiked beer samples. Recoveries averaged 90-104%, 94%, 84-87%, and 89% for aflatoxins B1, B2, G1, and G2, respectively, at levels of 9.7-133 ng B1, 46 ng B2, 35-140 ng G1, and 41 ng G2/L. Detection limits were 19-20 ng/L for aflatoxins B1 and G1 and 15-16 ng/L for aflatoxins B2 and G2 (signal-to-noise ratio = 3:1) obtained by using an excitation wavelength of 360 nm; at 340 nm these detection limits were lowered to about 2 ng/L. Analysis of 24 beer samples, the majority from the United States and Mexico, showed natural contamination of one sample of Mexican beer at 49 ng B1/L when determined at 360 nm excitation, but reanalysis of 23 of the samples using 340 nm excitation indicated that an additional 4 Mexican samples and one Brazilian sample contained aflatoxin B1 at low levels (< 10 ng/L).
采用免疫亲和柱净化和反相液相色谱法（LC），在三氟乙酸衍生化后进行荧光检测，测定啤酒中痕量水平的黄曲霉毒素B1、B2、G1和G2。为了从加标的啤酒样品中获得良好的黄曲霉毒素回收率，硅烷化小瓶对于蒸发步骤是必需的。在9.7 - 133 ng B1、46 ng B2、35 - 140 ng G1和41 ng G2/L的水平下，黄曲霉毒素B1、B2、G1和G2的回收率分别平均为90 - 104%、94%、84 - 87%和89%。在激发波长为360 nm时，黄曲霉毒素B1和G1的检测限为19 - 20 ng/L，黄曲霉毒素B2和G2的检测限为15 - 16 ng/L（信噪比 = 3:1）；在340 nm时，这些检测限降至约2 ng/L。对24个啤酒样品（大多数来自美国和墨西哥）的分析表明，在360 nm激发下测定时，一个墨西哥啤酒样品天然污染了49 ng B1/L，但使用340 nm激发对其中23个样品重新分析表明，另外4个墨西哥样品和1个巴西样品含有低水平（< 10 ng/L）的黄曲霉毒素B1。
