Poulsen R L, Knudsen J D, Petersen M B, Fuursted K, Espersen F, Frimodt-Møller N
Department of Research and Development in Microbiology, Statens Serum Institut, Copenhagen, Denmark.
APMIS. 1996 Jul-Aug;104(7-8):549-56. doi: 10.1111/j.1699-0463.1996.tb04910.x.
Four different diffusion tests used to detect penicillin resistance in Streptococcus pneumoniae were evaluated for 34 penicillin-susceptible pneumococci (MIC < 0.1 microgram/ml), 35 intermediate pencillin-resistant (MIC 0.1-1.0 microgram/ml) and 23 penicillin-resistant strains (MIC > 2 micrograms/ml). The 1 microgram oxacillin disk from AB Biodisk, the 1 microgram oxacillin Neo-Sensitabs from Rosco, the 5 micrograms penicillin Low Neo-Sensitabs and the penicillin E test were tested on Mueller-Hinton blood agar, PDM Antibiotic Sensitivity Medium II supplemented with blood, and Danish Blood Agar. MICs obtained by the agar dilution method were used as reference. The 1 microgram oxacillin AB Biodisk was able to separate all the penicillin-susceptible pneumococci correctly from those with reduced penicillin susceptibility (MIC > or = 0.1 microgram/ml), whereas use of the 1 microgram oxacillin Neo-Sensitabs resulted in high frequencies (14-29%) of intermediate penicillin-resistant strains interpreted as penicillin susceptible. The 5 micrograms penicillin Low Neo-Sensitabs proved completely useless for detecting penicillin resistance in pneumococci. High rates of agreement (82-93%) were found between the penicillin E test and the reference MIC determination method on all the tested media.
对34株青霉素敏感肺炎链球菌（MIC＜0.1微克/毫升）、35株青霉素中度耐药（MIC 0.1 - 1.0微克/毫升）和23株青霉素耐药菌株（MIC＞2微克/毫升），评估了用于检测肺炎链球菌青霉素耐药性的4种不同扩散试验。在穆勒 - 欣顿血琼脂、添加血液的PDM抗生素敏感性培养基II和丹麦血琼脂上，检测了AB生物盘的1微克苯唑西林纸片、罗斯科的1微克苯唑西林新药敏片、5微克青霉素低新药敏片和青霉素E试验。通过琼脂稀释法获得的MIC用作参考。1微克苯唑西林AB生物盘能够正确地将所有青霉素敏感肺炎链球菌与青霉素敏感性降低（MIC≥0.1微克/毫升）的菌株区分开，而使用1微克苯唑西林新药敏片时，被判定为青霉素敏感的中度青霉素耐药菌株的频率较高（14% - 29%）。5微克青霉素低新药敏片被证明完全无法用于检测肺炎链球菌的青霉素耐药性。在所有测试培养基上，青霉素E试验与参考MIC测定方法之间的一致性率较高（82% - 93%）。
