早期胸腺细胞发育所需的TCRβ链结构域。
Domains of the TCR beta-chain required for early thymocyte development.
作者信息
Jacobs H, Iacomini J, van de Ven M, Tonegawa S, Berns A
机构信息
Division of Molecular Genetics, The Netherlands Cancer Institute, Amsterdam, The Netherlands.
出版信息
J Exp Med. 1996 Nov 1;184(5):1833-43. doi: 10.1084/jem.184.5.1833.
Abstract
The T cell receptor beta (TCR beta) chain controls the developmental transition from CD4-CD8- to CD4+8+thymocytes. We show that the extracellular constant region and the transmembrane region, but not the variable domain or cytoplasmic tail of the TCR beta chain are required for this differentiation step. TCR beta mutant chains lacking the cytoplasmic tail can be found at the cell surface both in functional TCR/CD3 complexes and in a GPI-anchored monomeric form indicating that the cytoplasmic tail of the TCR beta chain functions as an ER retention signal. The concordance between cell surface expression of the mutant chains as TCR/CD3 complexes and their capacity to mediate thymocyte differentiation supports the CD3 mediated feedback model in which preTCR/CD3 complexes control the developmental transition from CD4-CD8- to CD4+CD8+thymocytes.
摘要
T细胞受体β（TCRβ）链控制着从CD4-CD8-胸腺细胞到CD4+8+胸腺细胞的发育转变。我们发现，这一分化步骤需要TCRβ链的细胞外恒定区和跨膜区，但不需要可变区或胞质尾。缺乏胞质尾的TCRβ突变链既能以功能性TCR/CD3复合物的形式存在于细胞表面，也能以糖基磷脂酰肌醇（GPI）锚定的单体形式存在，这表明TCRβ链的胞质尾起着内质网滞留信号的作用。突变链作为TCR/CD3复合物在细胞表面的表达与其介导胸腺细胞分化的能力之间的一致性，支持了CD3介导的反馈模型，即前TCR/CD3复合物控制着从CD4-CD8-胸腺细胞到CD4+CD8+胸腺细胞的发育转变。
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Int Immunol. 1994 Jul;6(7):995-1001. doi: 10.1093/intimm/6.7.995.
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