Kasai S, Sugimura K, Matsumoto K, Nishi N, Kishimoto T, Nakamura T
Department of Urology, Osaka City University Medical School, Japan.
Biochem Biophys Res Commun. 1996 Nov 12;228(2):646-52. doi: 10.1006/bbrc.1996.1710.
Hepatocyte growth factor (HGF) is a mesenchymal-derived growth factor which predominantly targets various epithelial and endothelial cells. We here investigated the biological function of HGF in rat dorsolateral prostatic tissue. HGF stimulated proliferation and DNA synthesis of prostatic epithelial cells in primary culture. c-Met/HGF receptor mRNA was specifically expressed in isolated epithelial cells. An immunohistochemical study revealed that HGF is located in prostatic stromal cells. When stromal cells were isolated and cultured, they produced significant amounts of HGF, which was further stimulated by prostaglandin E2, 12-O-tetradecanoylphorbol-13-acetate, and dibutyryl cyclic AMP, but not by testosterone. These results indicate that HGF is a stromal-derived potent mitogen for dorsolateral prostatic epithelial cells which may function as a paracrine regulator for growth of the prostatic glands.
肝细胞生长因子（HGF）是一种间充质来源的生长因子，主要作用于各种上皮细胞和内皮细胞。我们在此研究了HGF在大鼠背外侧前列腺组织中的生物学功能。HGF刺激原代培养的前列腺上皮细胞增殖和DNA合成。c-Met/HGF受体mRNA在分离的上皮细胞中特异性表达。免疫组织化学研究显示HGF位于前列腺基质细胞中。当分离并培养基质细胞时，它们会产生大量HGF，前列腺素E2、12-O-十四酰佛波醇-13-乙酸酯和二丁酰环磷酸腺苷可进一步刺激HGF产生，但睾酮不能。这些结果表明，HGF是一种基质来源的强效有丝分裂原，可作用于背外侧前列腺上皮细胞，可能作为旁分泌调节因子调节前列腺生长。
