Takahashi M, Ota S, Ogura K, Nakamura T, Omata M
2nd Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.
Biochem Biophys Res Commun. 1995 Nov 2;216(1):298-305. doi: 10.1006/bbrc.1995.2624.
We have recently established an in vitro primary culture system for esophageal epithelial cells, which enabled us to investigate the effect of hepatocyte growth factor (HGF) and other factors on esophageal restitution. HGF remarkably stimulated restitution of these cells. So did epidermal growth factor (EGF), though moderately. Restitution velocity of esophageal cells was remarkably higher than that of gastric epithelial cells. The expression of c-met, specific HGF receptor was demonstrated by the esophageal cells, suggesting that the effect of HGF was mediated by its specific receptor. The expression level of c-met mRNA was the same as that of gastric epithelial cells, as assessed by competitive RT-PCR technique. These results suggest that HGF might be involved in the repair process of esophageal mucosal damage.
我们最近建立了一种食管上皮细胞的体外原代培养系统，这使我们能够研究肝细胞生长因子（HGF）和其他因素对食管修复的影响。HGF显著刺激了这些细胞的修复。表皮生长因子（EGF）也有此作用，不过程度适中。食管细胞的修复速度明显高于胃上皮细胞。食管细胞显示出c-met（特异性HGF受体）的表达，表明HGF的作用是通过其特异性受体介导的。通过竞争性逆转录聚合酶链反应技术评估，c-met mRNA的表达水平与胃上皮细胞相同。这些结果表明，HGF可能参与食管黏膜损伤的修复过程。
