人类18号染色体的高密度辐射杂种图谱及18p的重叠群

High-density radiation hybrid map of human chromosome 18 and contig of 18p.

作者信息

Giacalone J, Li X, Lehrach H, Francke U

机构信息

Department of Genetics, Stanford University School of Medicine, California 94305, USA.

出版信息

Genomics. 1996 Oct 1;37(1):9-18. doi: 10.1006/geno.1996.0514.

DOI:10.1006/geno.1996.0514

PMID:8921364

Abstract

A high-resolution radiation hybrid map of human chromosome 18 has been developed by testing DNA samples of 92 radiation hybrids (RH) from a previously characterized chromosome 18-specific RH panel. Half of the 159 STS markers were tested on RH DNA amplified by primer extension preamplification. This map includes 20 genes and 95 polymorphic markers, most of which have heterozygosity frequencies greater than 65% and, therefore, allow integration with genetic maps. The framework map consists of 137 markers ordered with odds of 1000:1 or better and spaced on average 580 kb apart. A YAC contig map of 18p was generated independently by STS content mapping of YACs using the same primers as for RH mapping. The RH map and the contig map are concordant.

摘要

通过对来自先前鉴定的18号染色体特异性辐射杂种（RH）面板的92个辐射杂种（RH）的DNA样本进行检测，构建了人类18号染色体的高分辨率辐射杂种图谱。159个序列标签位点（STS）标记中的一半在通过引物延伸预扩增扩增的RH DNA上进行了检测。该图谱包括20个基因和95个多态性标记，其中大多数的杂合频率大于65%，因此能够与遗传图谱整合。框架图谱由137个标记组成，其排序的优势比为1000:1或更高，平均间距为580 kb。使用与RH图谱相同的引物，通过YAC的STS含量作图独立生成了18p的酵母人工染色体（YAC）重叠群图谱。RH图谱和重叠群图谱是一致的。