Collins J E, Cole C G, Smink L J, Garrett C L, Leversha M A, Soderlund C A, Maslen G L, Everett L A, Rice K M, Coffey A J
Sanger Centre, Hinxton, Cambridge, UK.
Nature. 1995 Sep 28;377(6547 Suppl):367-79. doi: 10.1038/377367a0.
We have constructed a high-resolution clone map of human chromosome 22 which integrates the available physical and genetic information, establishing a single consensus. The map consists of all classes of DNA landmarks ordered on 705 yeast artificial chromosomes (YACs) at an average landmark density of more than one per 70 kilobases. This map represents the practical limits of currently available YAC resources and provides the basis for determination of the entire gene content and genomic DNA sequence of human chromosome 22.
我们构建了人类22号染色体的高分辨率克隆图谱，该图谱整合了现有的物理和遗传信息，形成了单一的共识。该图谱由各类DNA界标组成，这些界标排列在705个酵母人工染色体（YAC）上，平均界标密度超过每70千碱基一个。此图谱代表了当前可用YAC资源的实际极限，并为确定人类22号染色体的全部基因内容和基因组DNA序列提供了基础。
