Christensen J P, Stenvang J P, Marker O, Thomsen A R
Institute of Medical Microbiology and Immunology, Panum Institute, Copenhagen, Denmark.
Int Immunol. 1996 Sep;8(9):1453-61. doi: 10.1093/intimm/8.9.1453.
Infection with lymphocytic choriomeningitis virus is associated with marked polyclonal activation of the CD8+ T cell subpopulation. In this report the cytokine production of virus-activated T cells is analyzed and the producing cell subset is characterized phenotypically. Coinciding with other parameters of cell-mediated immunity, splenic T cells appear which are able to release high amounts of IFN- gamma, but not IL-5, IL-10 or tumor necrosis factor-alpha upon short-term stimulation with anti-CD3 in vitro. A similar profile is observed analyzing T cells taken from an inflammatory site. Phenotypically, the main cytokine-producing cell subset is found to be CD8+ cells targeted for homing to inflammatory sites (VLA-4hiL-selectinlo) of which 30-40% were positive by intracellular staining for IFN-gamma. This subset also contains all T cells with a cytotoxic potential as measured by redirected killing. An enhanced cytotoxic potential as well as an increased capacity to produce IFN-gamma is observed for at least 2 months after infection and cell sorting analysis revealed that this could be ascribed to a long-standing increase in the frequency of CD8+ Pgp-1hi cells. Therefore, these results demonstrate that systemic virus infection may exert marked perturbation of the CD8+ T cell population resulting in generation of a long-lived subset of primed cells with important effector potential.
淋巴细胞性脉络丛脑膜炎病毒感染与CD8 + T细胞亚群的显著多克隆激活相关。在本报告中，分析了病毒激活的T细胞的细胞因子产生情况，并对产生细胞亚群进行了表型特征分析。与细胞介导免疫的其他参数一致，体外经抗CD3短期刺激后，脾脏中出现了能够释放大量干扰素-γ但不释放白细胞介素-5、白细胞介素-10或肿瘤坏死因子-α的T细胞。分析取自炎症部位的T细胞时也观察到类似的情况。从表型上看，主要的细胞因子产生细胞亚群是靶向归巢至炎症部位（VLA-4hiL-选择素lo）的CD8 +细胞，其中30-40%通过细胞内染色检测干扰素-γ呈阳性。该亚群还包含所有具有细胞毒性潜能的T细胞，这通过重定向杀伤来测定。感染后至少2个月观察到细胞毒性潜能增强以及产生干扰素-γ的能力增加，细胞分选分析表明这可归因于CD8 + Pgp-1hi细胞频率的长期增加。因此，这些结果表明全身性病毒感染可能对CD8 + T细胞群体产生显著扰动，导致产生具有重要效应潜能的长期存活的致敏细胞亚群。
