Feng C G, Bean A G, Hooi H, Briscoe H, Britton W J
Centenary Institute of Cancer Medicine and Cell Biology, Newtown, New South Wales, Australia 2042.
Infect Immun. 1999 Jul;67(7):3242-7. doi: 10.1128/IAI.67.7.3242-3247.1999.
Although it is well established that CD4(+) T cells are required for the protective immune response against tuberculosis (TB), there is some evidence that CD8(+) T cells are also involved in the host response to Mycobacterium tuberculosis. There is, however, a paucity of information on the pulmonary CD8(+) T-cell response during infection. We therefore have compared the changes in both CD8(+) and CD4(+) T cells following aerosol infection with M. tuberculosis. There was an observed delay between the peak of infection and the activated T-cell response in the lung. The kinetics of CD8(+) and CD4(+) T-cell responses in the lung were identical, both peaking at week 8, 4 weeks later than the peak of cellular response in draining lymph nodes. Similar changes in activation/memory phenotypes occurred on the pulmonary CD8(+) and CD4(+) T cells. Following in vitro restimulation, both subsets synthesized gamma interferon, a cytokine essential for controlling M. tuberculosis infection. Since lung CD8(+) T cells are actively expanded during aerosol M. tuberculosis infection, it is important that both CD8(+) and CD4(+) T cells be targeted in the design of future TB vaccines.
尽管已有充分证据表明，针对结核病（TB）的保护性免疫反应需要CD4(+) T细胞，但也有一些证据显示，CD8(+) T细胞也参与宿主对结核分枝杆菌的反应。然而，关于感染期间肺部CD8(+) T细胞反应的信息却很少。因此，我们比较了气溶胶感染结核分枝杆菌后CD8(+)和CD4(+) T细胞的变化。在感染高峰期与肺部活化T细胞反应之间观察到有延迟。肺部CD8(+)和CD4(+) T细胞反应的动力学相同，均在第8周达到峰值，比引流淋巴结中细胞反应的峰值晚4周。肺部CD8(+)和CD4(+) T细胞的活化/记忆表型发生了类似变化。体外再刺激后，两个亚群均合成γ干扰素，这是控制结核分枝杆菌感染所必需的一种细胞因子。由于在气溶胶感染结核分枝杆菌期间肺部CD8(+) T细胞会积极扩增，因此在未来结核病疫苗的设计中同时针对CD8(+)和CD4(+) T细胞很重要。
