Dykxhoorn D M, St Pierre R, Linn T
Department of Microbiology and Immunology, Faculty of Medicine, University of Western Ontario, London, Canada.
Gene. 1996 Oct 24;177(1-2):133-6. doi: 10.1016/0378-1119(96)00289-2.
A series of expression vectors have been developed which all contain an identical expression cassette comprised of the lacIq gene, the tac promoter, a multiple cloning site (MCS) and a downstream transcriptional terminator. This cassette has been inserted into four distinct plasmid backbones, each of which is from a separate incompatibility group and carries a different drug resistance gene. Therefore, different combinations of these expression plasmids can be stably maintained together.
已经开发出一系列表达载体，它们都包含一个相同的表达盒，该表达盒由lacIq基因、tac启动子、多克隆位点（MCS）和下游转录终止子组成。这个表达盒已被插入到四个不同的质粒骨架中，每个骨架都来自一个单独的不相容群，并携带不同的耐药基因。因此，这些表达质粒的不同组合可以稳定地共同维持。
