Warren W D, Phillips A M, Howells A J
Department of Biochemistry and Molecular Biology, Australian National University, Canberra, Australia.
Gene. 1996 Oct 24;177(1-2):149-53. doi: 10.1016/0378-1119(96)00291-0.
A transcription unit was identified in the 43E polytene band region of the second chromosome of Drosophila melanogaster (Dm) whose putative translation product has 85% amino acid (aa) identity with the B subunit of the calcineurin protein (CnB) from humans. Unlike the previously described intronless Dm CnB gene homologue, which is located within the 4F band region of the X chromosome, the coding region of this second CnB is found to be interrupted by three introns. Conceptual translation of both Dm CnB genes predict proteins of identical size that are 98% identical in aa sequence. Northern blot analyses indicate that Dm pupae and adults express two different CnB-encoding transcripts that are differentially regulated.
在黑腹果蝇（Dm）第二条染色体的43E多线带区域鉴定出一个转录单位，其推定的翻译产物与人类钙调神经磷酸酶蛋白（CnB）的B亚基具有85%的氨基酸（aa）同一性。与先前描述的位于X染色体4F带区域内的无内含子Dm CnB基因同源物不同，该第二个CnB的编码区域被三个内含子中断。两个Dm CnB基因的概念性翻译预测大小相同的蛋白质，其aa序列具有98%的同一性。Northern印迹分析表明，Dm蛹和成虫表达两种不同的、受差异调节的CnB编码转录本。
