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果蝇黑腹eIF-2α基因的分离与鉴定，该基因编码翻译起始因子eIF-2的α亚基。

Isolation and characterization of the Drosophila melanogaster eIF-2 alpha gene encoding the alpha subunit of translation initiation factor eIF-2.

作者信息

Qu S, Cavener D R

机构信息

Department of Molecular Biology, Vanderbilt University, Nashville, TN 37235.

出版信息

Gene. 1994 Mar 25;140(2):239-42. doi: 10.1016/0378-1119(94)90550-9.

DOI:10.1016/0378-1119(94)90550-9
PMID:8144032
Abstract

Genomic and cDNA clones encoding the Drosophila melanogaster alpha-subunit of translational initiation factor 2 (eIF-2 alpha) were isolated. The D. melanogaster eIF-2 alpha gene encodes a 341 amino-acid (aa) protein that shares 57 and 44% identity to its human and yeast homologues, respectively. The regulatory phosphorylation site at Ser50 is embedded in a segment of 19 conserved aa residues. Analysis of the genomic DNA and cDNA clones indicated that eIF-2 alpha is a single-copy gene and its coding region is interrupted by a 260-bp intron. The D. melanogaster eIF-2 alpha mRNA is 1350 nt in length and is expressed throughout development.

摘要

分离出了编码黑腹果蝇翻译起始因子2（eIF - 2α）α亚基的基因组和cDNA克隆。黑腹果蝇eIF - 2α基因编码一种341个氨基酸（aa）的蛋白质，分别与其人类和酵母同源物具有57%和44%的同一性。Ser50处的调节性磷酸化位点位于19个保守氨基酸残基的片段中。对基因组DNA和cDNA克隆的分析表明，eIF - 2α是一个单拷贝基因，其编码区被一个260 bp的内含子中断。黑腹果蝇eIF - 2α mRNA长度为1350 nt，在整个发育过程中均有表达。

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