Wawrzos I, Kitagawa Y, Kołoczek H
Agriculture University, Department of Biochemistry, Cracow, Poland.
Acta Biochim Pol. 1996;43(3):481-7.
The immunodiffusion cross-reactivity and competitive inhibition ELISA assays were used for immunological differentiation of latent form, cleaved form and guanidinium hydrochloride (GuHCl) induced polymer of human alpha 1-proteinase inhibitor (alpha 1-PI). Under the conditions studied, the differences between latent form and GuHCl-induced polymers of the inhibitor in terms of immunological response were estimated to amount to about 30% and differences between latent and cleaved alpha 1-PI to about 50%. The immunodiffusion and ELISA data for citrate-induced polymers suggest that in their structure the latent molecule is involved. On the basis of competitive inhibition data, we suggest that the alpha 1-PI protein polymerisation involves insertion of the reactive-site loop (RSL) into the A-sheet under mild conditions and that in the latent form of the inhibitor RSL is incompletely inserted into the A-sheet.
免疫扩散交叉反应和竞争性抑制酶联免疫吸附测定法被用于对人α1-蛋白酶抑制剂（α1-PI）的潜伏形式、裂解形式以及盐酸胍（GuHCl）诱导的聚合物进行免疫分化。在所研究的条件下，抑制剂的潜伏形式与GuHCl诱导的聚合物在免疫反应方面的差异估计约为30%，而潜伏型和裂解型α1-PI之间的差异约为50%。柠檬酸盐诱导聚合物的免疫扩散和酶联免疫吸附测定数据表明，潜伏分子参与了其结构。基于竞争性抑制数据，我们认为α1-PI蛋白聚合涉及在温和条件下反应位点环（RSL）插入A片层，并且在抑制剂的潜伏形式中，RSL未完全插入A片层。
