3' Noncoding sequences of the CTA 1 gene enhance expression of the recombinant serine protease inhibitor, CPTI II, in Saccharomyces cerevisiae.
作者信息
Stankiewicz M, Rempoła B, Fikus M
机构信息
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland.
出版信息
Acta Biochim Pol. 1996;43(3):525-9.
PMID:8922037
Abstract
Expression of the gene coding for the recombinant trypsin inhibitor, CPTI II, was enhanced tenfold when yeast transcription terminating sequences were added to the expression cassette of the pJK6 yeast vector. The yield was further increased about 20% in the BJ5464 yeast strain, defective in vacuolar proteases.
摘要
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