[酿酒酵母ADE12基因，编码腺苷酸琥珀酸合成酶（EC 6.3.4.4）。克隆、测序、表达及超量生产]
[Saccharomyces cerevisiae ADE12 gene, coding for adenylosuccinate synthetase (EC 6.3.4.4). Cloning, sequencing, expression, and superproduction].
作者信息
Andreĭchuk Iu V, Shabes A V, Ryzhova T A, Kotova I A, Domkin V D
出版信息
Mol Gen Mikrobiol Virusol. 1995 Jan-Mar(1):21-8.
PMID:7603488
Abstract
The cloning and sequencing of Saccharomyces cerevisiae ADE12 gene encoding the structure of adenylsuccinate synthetase (ASS) are reported for the first time. Comparative analysis of all known ASS sequences was carried out. Regulation of ADE12 gene expression by exogenic adenine was carried out. The properties of ASS which was isolated and purified from overproducing yeast strain were examined.
摘要
首次报道了酿酒酵母中编码腺苷酸琥珀酸合成酶（ASS）结构的ADE12基因的克隆和测序。对所有已知的ASS序列进行了比较分析。研究了外源腺嘌呤对ADE12基因表达的调控。检测了从过量表达酵母菌株中分离纯化得到的ASS的性质。
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