Fawl R L, Gesser R M, Valyi-Nagi T, Fraser N W
The Wistar Institute, Philadelphia, PA 19104, USA.
J Gen Virol. 1996 Nov;77 ( Pt 11):2781-6. doi: 10.1099/0022-1317-77-11-2781.
It was previously reported that administration of cadmium (Cd) to CBA mice latently infected with herpes simplex virus (HSV) results in a high incidence of virus reactivation in vivo. In the present study, Cd-inducible reactivation was used to compare CBA with four other laboratory mouse strains. HSV reactivation, as measured by the recovery of infectious particles from latently infected trigeminal ganglia following Cd treatment, occurred predominantly in the CBA strain and was almost entirely absent from other strains tested. There was no correlation of strain-dependent Cd toxicity with the recovery of infectious virus. In situ examination of Cd-treated ganglia from latently infected CBA and BALB/c mice revealed that viral antigens were expressed exclusively in CBA specimens, but that viral replicative transcripts were expressed in both strains, although more strongly in CBA than in BALB/c specimens. We conclude that Cd treatment had induced reactivation of HSV from both mouse strains, and that the reactivation process was completed in CBA but not in BALB/c mice.
先前有报道称，给潜伏感染单纯疱疹病毒（HSV）的CBA小鼠施用镉（Cd）会导致体内病毒再激活的高发生率。在本研究中，利用镉诱导的再激活来比较CBA小鼠与其他四种实验室小鼠品系。通过在镉处理后从潜伏感染的三叉神经节中回收感染性颗粒来测量HSV再激活，其主要发生在CBA品系中，而在其他测试品系中几乎完全不存在。品系依赖性镉毒性与感染性病毒的回收之间没有相关性。对来自潜伏感染的CBA和BALB/c小鼠的经镉处理的神经节进行原位检查发现，病毒抗原仅在CBA标本中表达，但病毒复制转录本在两个品系中均有表达，尽管在CBA中比在BALB/c标本中表达更强。我们得出结论，镉处理诱导了两种小鼠品系中HSV的再激活，并且再激活过程在CBA小鼠中完成，但在BALB/c小鼠中未完成。
