Gründemann D, Schömig E
University of Heidelberg, Heidelberg, Germany.
Biotechniques. 1996 Nov;21(5):898-903. doi: 10.2144/96215rr02.
Preparative gel electrophoresis of double-stranded DNA usually includes staining the gel with ethidium bromide followed by illumination with ultraviolet (UV-B) light. In this report, DNA isolated from agarose gels was found to be a poor substrate for in vitro transcription, transformation of E. coli and PCR. Inhibition was not caused by enzyme-inhibiting impurities in the agarose gel, but was induced by a standard transilluminator fitted with 312-nm tubes. Interestingly, it was possible to protect the DNA against UV damage by the addition of cytidine or guanosine to the electrophoresis buffer.
双链DNA的制备性凝胶电泳通常包括用溴化乙锭对凝胶进行染色，然后用紫外（UV-B）光照射。在本报告中，发现从琼脂糖凝胶中分离出的DNA是体外转录、大肠杆菌转化和聚合酶链反应（PCR）的不良底物。抑制作用不是由琼脂糖凝胶中的酶抑制杂质引起的，而是由装有312纳米灯管的标准透射仪诱导的。有趣的是，通过在电泳缓冲液中添加胞苷或鸟苷，可以保护DNA免受紫外线损伤。
