人重组干扰素α-2a抑制无壳培养中鸡胚绒毛尿囊膜血管生成。

Human recombinant interferon alpha-2a inhibits angiogenesis of chick area vasculosa in shell-less culture.

作者信息

Ribatti D, Vacca A, Iurlaro M, Ria R, Roncali L, Dammacco F

机构信息

Institute of Human Anatomy, Histology and Embryology, University of Bari, Italy.

出版信息

Int J Microcirc Clin Exp. 1996 Jul-Aug;16(4):165-9. doi: 10.1159/000179169.

DOI:10.1159/000179169

PMID:8923148

Abstract

In vivo suppression of neovascularization by in situ administration of human recombinant interferon alpha 2a (hrIFN-alpha 2a) was tested on the chick embryo area vasculosa (AV). Methylcellulose discs, each containing 5 IU of hrIFN-alpha 2a were implanted onto the AV at Hamburger-Hamilton (HH) stage 13, and inhibition of blood vessel growth was morphometrically evaluated between HH stages 20 and 27. Our results show: (i) a decreased extent of the AV; (ii) a decreased AV total vessel length; (iii) modified percent ratios of different classes of AV vessels having a definite length. The observed effects of treating with hrIFN-alpha 2a are likely attributable to inhibition of endothelial cell proliferation.

摘要

通过在鸡胚血管区（AV）原位给予人重组干扰素α 2a（hrIFN-α 2a）对体内新血管形成的抑制作用进行了测试。将每个含有5国际单位hrIFN-α 2a的甲基纤维素圆盘在汉伯格-汉密尔顿（HH）第13阶段植入AV，并在HH第20至27阶段之间通过形态计量学评估血管生长的抑制情况。我们的结果表明：（i）AV范围减小；（ii）AV总血管长度减小；（iii）具有确定长度的不同类别的AV血管的百分比比例改变。观察到的用hrIFN-α 2a治疗的效果可能归因于内皮细胞增殖的抑制。