Allen W E, Wilson D J
School of Biomedical Science, Queen's University of Belfast, UK.
J Anat. 1993 Dec;183 ( Pt 3)(Pt 3):579-85.
The rate and pattern of growth as well as vessel ultrastructure of the area vasculosa were examined in the chick. The embryos were grown in shell-less culture after 3 d in ovo and staged according to Hamburger & Hamilton (1951) and the rate of increase in the diameter of the area vasculosa was measured. This revealed an increase in the area vasculosa diameter of 0.4 +/- 0.02 mm h-1 (n = 62) for embryos between stages 15 and 20. To determine the growth pattern of the sinus terminalis (the advancing edge of the area vasculosa), a marked length of the sinus was photographed at hourly intervals over a period of 9 h. It was found that this vessel grows by new vessels forming external to the sinus in the form of parallel plexuses, one of which then replaces the original sinus as the major route of bloodflow. Ultrastructurally the capillaries of the area vasculosa were simple tubes of endothelial cells, lacking a basement membrane. The endothelial cell cytoplasm contained only a few organelles, mainly mitochondria and rough endoplasmic reticulum. These findings indicate that the chick area vasculosa capillaries bear similar structural and growth characteristics to those associated with tumour angiogenesis and suggest that they may prove to be a useful model system for studying the factors involved in pathological angiogenesis.
在鸡胚中研究了血管区的生长速率和模式以及血管超微结构。胚胎在卵内发育3天后进行无壳培养，并根据Hamburger和Hamilton（1951）的标准进行分期，测量血管区直径的增加速率。结果显示，15至20期胚胎的血管区直径增加速率为0.4±0.02 mm h-1（n = 62）。为了确定终窦（血管区的前沿）的生长模式，在9小时内每隔一小时拍摄一次终窦的一段标记长度。发现该血管通过在窦外部以平行丛状形式形成新血管而生长，其中之一随后取代原始窦成为主要血流途径。在超微结构上，血管区的毛细血管是由内皮细胞构成的简单管状物，缺乏基底膜。内皮细胞胞质中仅含有少量细胞器，主要是线粒体和粗面内质网。这些发现表明，鸡胚血管区的毛细血管具有与肿瘤血管生成相关的相似结构和生长特征，并表明它们可能被证明是研究病理性血管生成相关因素的有用模型系统。
