Robinson S, Miller R
Department of Neurosciences, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106, USA.
Mol Cell Neurosci. 1996;8(1):38-52. doi: 10.1006/mcne.1996.0042.
The timing of oligodendrogenesis depends on the specific location within the central nervous system, suggesting the local environment influences oligodendrocyte precursor proliferation. Spinal cord and optic nerve oligodendrocyte precursors both proliferate in response to platelet-derived growth factor (PDGF). Here we show that neurotrophin-3 (NT-3) enhanced PDGF-induced proliferation of optic nerve oligodendrocyte precursors, and these cells were labeled by an anti-trkC antibody. By contrast, NT-3 did not enhance PDGF-induced proliferation of spinal cord oligodendrocyte precursors, and these cells were not labeled by an anti-trkC antibody. Furthermore, PDGF-induced oligodendrocyte precursor proliferation was greater in spinal cord than in optic nerve cultures. The difference in NT-3 response between spinal cord and optic nerve oligodendrocyte precursors appears cell intrinsic, while the enhanced PDGF-induced proliferation of spinal cord oligodendrocyte precursors appears environmentally regulated. The spinal cord PDGF proliferation-enhancing activity may provide a mechanism to allow temporal and spatial regulation of gliogenesis.
少突胶质细胞生成的时间取决于中枢神经系统内的特定位置，这表明局部环境会影响少突胶质细胞前体细胞的增殖。脊髓和视神经的少突胶质细胞前体细胞都会对血小板衍生生长因子（PDGF）产生增殖反应。在此我们表明，神经营养因子-3（NT-3）增强了PDGF诱导的视神经少突胶质细胞前体细胞的增殖，并且这些细胞被抗trkC抗体标记。相比之下，NT-3并未增强PDGF诱导的脊髓少突胶质细胞前体细胞的增殖，并且这些细胞未被抗trkC抗体标记。此外，PDGF诱导的少突胶质细胞前体细胞增殖在脊髓中比在视神经培养物中更显著。脊髓和视神经少突胶质细胞前体细胞对NT-3反应的差异似乎是细胞内在的，而脊髓少突胶质细胞前体细胞中PDGF诱导的增殖增强似乎受环境调节。脊髓中PDGF增殖增强活性可能提供一种机制，以实现胶质细胞生成的时空调节。
