Engel U, Wolswijk G
Ludwig Institute for Cancer Research, London, England.
Glia. 1996 Jan;16(1):16-26. doi: 10.1002/(SICI)1098-1136(199601)16:1<16::AID-GLIA3>3.0.CO;2-9.
We have analysed in detail the properties of oligodendrocyte-type-2 astrocyte (O-2A) progenitor cells derived from the spinal cords of adult rats to gain further insights into the mechanisms that control the generation of oligodendrocytes in the healthy and demyelinated adult central nervous systems (CNS). When O-2A progenitor cells from adult spinal cord are exposed in vitro to the AA homodimeric form of platelet-derived growth factor (PDGF-AA), they express a unipolar morphology, an O4-positive, vimentin-negative antigenic phenotype, divide at slow rates, and appear to generate oligodendrocytes by asymmetric division and differentiation. Furthermore, exposure of these cells to PDGF-AA is sufficient to stimulate their proliferation at clonal density. When adult spinal cord O-2A progenitor cells are exposed simultaneously to PDGF-AA and basic fibroblast growth factor (PDGF/bFGF), they are almost completely inhibited from differentiating into oligodendrocytes, divide more rapidly than cells treated with PDGF-AA, and express a bipolar morphology and an O4-negative, vimentin-positive antigenic phenotype. These findings indicate that adult spinal cord O-2A progenitor cells resemble in many aspects their well-characterised adult optic nerve counterparts. In addition, evidence is presented to indicate that neurotrophin-3 (NT-3) is not mitogenic for adult spinal cord O-2A progenitor cells and that it does not enhance their proliferative response to PDGF-AA or PDGF/bFGF. Since relatively large numbers of O-2A progenitor cells can be obtained from adult spinal cord, it should facilitate the further characterisation of these cells.
我们详细分析了源自成年大鼠脊髓的少突胶质细胞-2型星形胶质细胞（O-2A）祖细胞的特性，以进一步深入了解在健康和脱髓鞘的成年中枢神经系统（CNS）中控制少突胶质细胞生成的机制。当来自成年脊髓的O-2A祖细胞在体外暴露于血小板衍生生长因子（PDGF-AA）的AA同二聚体形式时，它们呈现单极形态，具有O4阳性、波形蛋白阴性的抗原表型，分裂速度缓慢，并且似乎通过不对称分裂和分化产生少突胶质细胞。此外，将这些细胞暴露于PDGF-AA足以以克隆密度刺激其增殖。当成年脊髓O-2A祖细胞同时暴露于PDGF-AA和碱性成纤维细胞生长因子（PDGF/bFGF）时，它们几乎完全被抑制分化为少突胶质细胞，比用PDGF-AA处理的细胞分裂更快，并呈现双极形态和O4阴性、波形蛋白阳性的抗原表型。这些发现表明，成年脊髓O-2A祖细胞在许多方面与其特征明确的成年视神经对应细胞相似。此外，有证据表明神经营养因子-3（NT-3）对成年脊髓O-2A祖细胞没有促有丝分裂作用，并且它不会增强它们对PDGF-AA或PDGF/bFGF的增殖反应。由于可以从成年脊髓获得相对大量的O-2A祖细胞，这应该有助于对这些细胞进行进一步的表征。
