Alt A, Reinhardt G
Institut für Rechtsmedizin, Universität Ulm.
Blutalkohol. 1996 Jul;33(4):209-14.
The most commonly used methods for the determination of blood alcohol are the Head-Space Gas Chromatography (HSGC) and the ADH-Method. Abbott offers now a REA-ethanol Assay for the AXSYM-System. The accuracy of the 3 analytical procedural were checked by a method comparison. This involved blood alcohol concentration of 347 serum samples being determined by means of all 3 methods. The deviation of the results of double analysis within the respective method, the difference of the values when using 2 measuring methods each (HSGC-ADH, ADH-AXSYM, HSGC-AXSYM) and the standard deviation of the measurements from HSGC-AXSYM and HSGC-ADH were determined. The REA Ethanol Assay, which was developed for diagnostic and therapeutic questions in cases of alcohol intoxication works with an accuracy which suffices entirely for this task designation. However, the fixed regulation for determining blood alcohol in forensic use cannot be observed fully.
测定血液酒精含量最常用的方法是顶空气相色谱法（HSGC）和乙醇脱氢酶法（ADH法）。雅培公司目前为AXSYM系统提供了一种REA乙醇检测方法。通过方法比较来检验这三种分析程序的准确性。这涉及用所有三种方法测定347份血清样本的血液酒精浓度。确定了各方法内双份分析结果的偏差、每种使用两种测量方法时（HSGC - ADH、ADH - AXSYM、HSGC - AXSYM）的值的差异以及HSGC - AXSYM和HSGC - ADH测量的标准偏差。为酒精中毒病例的诊断和治疗问题而开发的REA乙醇检测方法的准确度完全满足此项任务要求。然而，在法医用途中测定血液酒精的固定规定无法完全遵守。
