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[在暗修复系统中存在缺陷的噬菌斑微生物突变体]

[Mutants of the plaque microbe defective in the dark repair system].

作者信息

Temiralieva G A

出版信息

Genetika. 1977;13(3):518-23.

PMID:892436
Abstract

The mutants of plague bacteria deficient in dark repair are described. They are sensitive to UV, mitomycin C, polymixin. Mutants have lost the ability to host cell reactivation in phage T7. The data obtained have shown that the addition of caffeine to the plating medium used to assay post-irradiation viability of UV-sensitive mutants did not influence the survival. Data are also given concerning virulence determinants, biochemical and other properties. The mutants formed P- colonies on hemine medium and Ca+ cells on Higuchi-Smith medium. They are avirulent for albino mice.

摘要

描述了缺乏暗修复能力的鼠疫杆菌突变体。它们对紫外线、丝裂霉素C、多粘菌素敏感。突变体失去了在噬菌体T7中进行宿主细胞复活的能力。所获得的数据表明,在用于检测紫外线敏感突变体辐射后活力的平板培养基中添加咖啡因不会影响存活率。还给出了关于毒力决定因素、生化及其他特性的数据。这些突变体在血氨酸培养基上形成P菌落,在Higuchi-Smith培养基上形成Ca+细胞。它们对白化小鼠无毒力。

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