Sevan'kaev A V, Luchnik N V
Genetika. 1977;13(3):524-32.
Cultures of human lymphocytes obtained from blood of healthy adult donors were irradiated at different doses of gamma-rays (60Co), and irradiated cells were analysed at metaphase in 50 hours after the irradiation. The effect (total yield of aberrations of chromosome type, or total yield of exchange type aberrations) produced by the lowest dose (5 r) appears to be statistically significant in a sample of 1500 cells. Within usual dose-range (25--400 r) both parabolic and linear-quadratic equations give a satisfactory fit of experimental data (dicentrics, fragments, or all aberration of the chromosome type). Low doses of gamma-rays produce, however, more aberrations than expected, if one extrapolates dose-effect curves from higher doses. Both equations should be considered therefore just as empirical equations. Dicentrics show a plateau at low doses (10--30 r) which appear to be statistically significant. Some indications are obtained that the total number of chromosome-type aberrations is a more reliable criterion of cytogenetic damage than usually accepted yield of dicentrics and rings.
从健康成年供体血液中获取的人类淋巴细胞培养物，用不同剂量的γ射线（60Co）进行照射，并在照射后50小时的中期对照射后的细胞进行分析。在1500个细胞的样本中，最低剂量（5伦琴）产生的效应（染色体型畸变的总产量或交换型畸变的总产量）在统计学上似乎具有显著意义。在通常的剂量范围内（25 - 400伦琴），抛物线方程和线性二次方程都能很好地拟合实验数据（双着丝粒、片段或染色体型的所有畸变）。然而，如果从较高剂量外推剂量效应曲线，低剂量的γ射线产生的畸变比预期的更多。因此，这两个方程都应仅被视为经验方程。双着丝粒在低剂量（10 - 30伦琴）时显示出一个平台期，这在统计学上似乎具有显著意义。有迹象表明，染色体型畸变的总数是比通常所接受的双着丝粒和环状体产量更可靠的细胞遗传学损伤标准。
