Thongpraditchote S, Matsumoto K, Temsiririrkkul R, Tohda M, Murakami Y, Watanabe H
Division of Pharmacology, Research Institute for Wakan-Yaku (Oriental Medicines), Toyama Medical and Pharmaceutical University, Japan.
Biol Pharm Bull. 1996 Mar;19(3):379-83. doi: 10.1248/bpb.19.379.
The effects of Pluchea indica Less root extract (PI-E) on locomotor activity and pentobarbital-induced sleep, social isolation-induced aggressive behavior, motor coordination in the rotarod test, pentylenetetrazole-induced convulsion and nociceptive responses in the tail-pinch test were examined in mice. Socially isolated mice showed higher locomotor activity and shorter duration of pentobarbital sleep than group-housed mice. PI-E (50-100 mg/kg, p.o.) significantly decreased locomotor activity and prolonged pentobarbital sleep in a dose-dependent manner in isolated mice but not in group-housed mice. At a large dose (400 mg/kg, p.o.), PI-E not only decreased locomotor activity but also prolonged pentobarbital sleep in group-housed mice. The reference drug diazepam, at 0.5 mg/kg, also suppressed the locomotor activity in isolated mice but not in group-housed mice. Moreover, diazepam, at 0.1 and 0.5 mg/kg, significantly prolonged pentobarbital sleep in both isolated mice and group-housed mice. The effects of PI-E and diazepam on pentobarbital sleep in isolated mice were significantly attenuated by flumazenil (1 mg/kg, i.v.). PI-E (50-100 mg/kg), as well as diazepam (0.5-5 mg/kg, p.o.), dose-dependently suppressed social isolation-induced aggressive behavior, but it had no effect on pentylenetetrazole-induced convulsion, motor coordination in the rotarod test, or nociceptive response in the tail pinch test in group-housed mice. These results suggest that PI-E attenuates pathophysiological changes caused by social isolation stress in mice, and that the GABAergic system is partly involved in the action of PI-E on a social isolation-induced decrease in pentobarbital sleep.
在小鼠中检测了印度艾纳香根提取物（PI-E）对自发活动、戊巴比妥诱导睡眠、社会隔离诱导攻击行为、转棒试验中的运动协调性、戊四氮诱导惊厥以及夹尾试验中伤害性反应的影响。与群居小鼠相比，社会隔离小鼠表现出更高的自发活动和更短的戊巴比妥睡眠时间。PI-E（50-100mg/kg，口服）能以剂量依赖方式显著降低隔离小鼠的自发活动并延长戊巴比妥睡眠时间，但对群居小鼠无此作用。大剂量（400mg/kg，口服）时，PI-E不仅能降低群居小鼠的自发活动，还能延长其戊巴比妥睡眠时间。参比药物地西泮，0.5mg/kg时，也能抑制隔离小鼠的自发活动，但对群居小鼠无效。此外，地西泮，0.1和0.5mg/kg时，能显著延长隔离小鼠和群居小鼠的戊巴比妥睡眠时间。氟马西尼（1mg/kg，静脉注射）能显著减弱PI-E和地西泮对隔离小鼠戊巴比妥睡眠的影响。PI-E（50-100mg/kg）以及地西泮（0.5-5mg/kg，口服）能剂量依赖地抑制社会隔离诱导的攻击行为，但对群居小鼠的戊四氮诱导惊厥、转棒试验中的运动协调性或夹尾试验中的伤害性反应无影响。这些结果表明，PI-E能减轻小鼠社会隔离应激引起的病理生理变化，且γ-氨基丁酸能系统部分参与了PI-E对社会隔离诱导的戊巴比妥睡眠减少的作用。
